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Suppression of NF-κB by Dieckol Extracted from Ecklonia cava Negatively Regulates LPS Induction of Inducible Nitric Oxide Synthase Gene

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Abstract

Dieckol, extracted from brown algae, Ecklonia cava, is suggested to elicit anti-inflammatory or anti-tumorigenic activities. However, dieckol-mediated regulatory mechanism for inflammatory response still remains elusive. Here, we show that dieckol suppressed lipopolysaccharide (LPS)-induced inducible nitric oxide synthase (iNOS) expression in mouse leukemic macrophage Raw264.7 cells. Also, dieckol decreased LPS-induced both nitric oxide (NO) production and iNOS promoter-driven transcriptional activity in a dose-dependent manner. On the other hand, LPS-mediated NF-κB activity was inhibited by dieckol treatment. Moreover, results revealed that dieckol diminished LPS-mediated p65 nuclear translocation or IκBα phosphorylation dose-dependently, and reduced LPS-induced phosphorylation of mitogen-activated protein kinases (MAPKs), significantly p38MAPK. Collectively, these findings suggest that dieckol acts as a negative regulator of LPS-mediated iNOS induction through suppression of NF-κB activity, implying a mechanistic role of dieckol in regulation of inflammatory response.

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Acknowledgments

We thank lab members for their helpful suggestions and critical comments on the manuscript.

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Authors and Affiliations

  1. Department of Biochemistry, College of Medicine, Konyang University, 685 Gasuwon-dong, Seo-gu, Daejeon, 302-832, South Korea

    Hye-Jin Choi, Jung-Hwan Park, Kyung Bok Lee & Sang-Muk Oh

  2. Department of Applied Chemistry, Hanbat National University, 16-1 Dukmyung-dong, Yuseong-gu, Daejeon, 305-719, South Korea

    Bong Ho Lee

  3. Department of Biology, College of Medicine, Konyang University, 685 Gasuwon-dong, Seo-gu, Daejeon, 302-832, South Korea

    Hee Youn Chee

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  1. Hye-Jin Choi
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  2. Jung-Hwan Park
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Correspondence to Sang-Muk Oh.

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Choi, HJ., Park, JH., Lee, B.H. et al. Suppression of NF-κB by Dieckol Extracted from Ecklonia cava Negatively Regulates LPS Induction of Inducible Nitric Oxide Synthase Gene. Appl Biochem Biotechnol 173, 957–967 (2014). https://doi.org/10.1007/s12010-014-0910-6

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  • DOI: https://doi.org/10.1007/s12010-014-0910-6

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