Abstract
To evaluate the degree of exposure and to distinguish the contributions of respirable amphibole fibers (both asbestos and non-asbestos) from natural and anthropogenic sources, we investigated their burden in the lungs of sentinel animals. Lung samples of sentinel animals are more easily and continuously available in comparison to humans and are not affected by human variables such as occupational exposure and smoking habit. Fiber identification and quantification have been performed using a scanning electron microscope (SEM) with an energy-dispersive spectrometer (EDS). Results for detected amphibole fibers (both asbestos and non-asbestos) were obtained from six studied areas in Northwest Italy (Piedmont) and correlated to lithological and urbanization data. Detected amphibole fiber species have been classified as asbestos minerals occur naturally (NOA) or to anthropogenically sourced asbestos (ASA). This pilot study is a potentially useful tool for the evaluation of the distribution and sources of respirable inorganic fibers. The model should be applicable in any areas where lithological and anthropogenic characteristics are known.
Similar content being viewed by others
References
Adib G, Labrèche F, De Guire L, Dion C, Dufresne AD (2013) Short, fine and WHO asbestos fibers in the lungs of Quebec workers with an asbestos-related disease. Am J Ind Med 56:1001–1014
Arpa Piemonte (2008) Amianto naturale in Piemonte. https://www.arpa.piemonte.gov.it/pubblicazioni-2/pubblicazioni-anno-2008/pdfamiantonaturale.pdf
Arpa Piemonte (2011) Mappatura delle coperture in cemento amianto. http://webgis.arpa.piemonte.it/w-metadoc/tumbnail
ATSDR (Agency for Toxic Substances and Disease Registry) (2002) Report on the Expert Panel on Health Effects of Asbestos and Synthetic Vitreous Fibers: the influence of fiber length. New York, NY: Expert Panel on Health Effects of Asbestos and Synthetic Vitreous Fibers; October 29–30, 2002
Aust AE, Cook PM, Dodson RF (2011) Morphological and chemical mechanisms of elongated mineral particle toxicities. J Toxicol Environ Health B Crit Rev 14:40–75
Baris I, Simonato L, Artvinli M, Pooley F, Saracci R, Skidmore J et al (1987) Epidemiological and environmental evidence of the health effects of exposure to erionite fibres: a four-year study in the Cappadocian region of Turkey. Int J Cancer 39:10–17
Belluso E, Compagnoni R, Ferraris G (1994) Occurrence of asbestiform minerals in the serpentinites of the Piemonte Zone, Western Alps. Giornata di studio in ricordo del Prof. S. Zucchetti, Politecnico di Torino 57–66
Belluso E, Ferraris G, Alberico A (1997) Amianto, la componente ambientale: dove, quali e come sono gli amianti nelle Alpi? In: L’amianto: dall’ambiente di lavoro all’ambiente di vita. Nuovi indicatori per futuri effetti. Fondazione S. Maugeri, IRCCS, Pavia – I documeti 12:167–174
Belluso E, Bellis D, Fornero E, Capella S, Ferraris G, Coverlizza S (2006) Assessment of inorganic fiber burden in biological samples by scanning electron microscopy-energy dispersive spectroscopy. Microchim Acta 155:95–100
Centeno JA, Mullick FG, Ishak KG, Franks TJ, Burke AP (2005) Environmental pathology. In: Selinus O, Alloway B, Centeno JA, Finkelman RB, Fuge R, Lindh U, Smedley P (eds) Essentials of medical geology. Burlington, USA, pp. 563–594
Chemical Safety In: Environmental Health Criteria, Geneva, Switzerland
Comba P, Bianchi F, Fazzo L, Martina L, Menegozzo M, Minichelli F, Mitis F, Musmeci L, Pizzuti R, Santoro M, Trinca S, Martuzzi M (2006) Cancer mortality in an area of Campania (Italy) characterized by multiple toxic dumping sites. Ann N Y Acad Sci 1076:49–461
Constantopoulos SH, Saratzis N, Kontogiannis D, Karantanas A, Goudevenos JA, Katsiotis P (1987) Tremolite whitewashing and pleural calcifications. Chest 92:709–712
De Nardo P, Bruni B, Paletti L, Pasetto R, Siriani B (2004) Pulmunary fibre burden in sheep living in the Biancavilla area (Sicily): preliminary results. Sci Total Environ 325:51–58
De Vuyst P, Karjalainen A, Dumotier P, Pairon JC, Monsò E, Brochard P, Teschler H, Tossavainen A, Gibbs A (1998) Guidelines for mineral fibre analyses in biological samples: report of the ERS Working Group. Eur Respir J 11:1416–1426
Directive 2003/18/EC of the European Parliament and of the Council of 27 March 2003 Amending Council Directive 83/477/EEC on the protection of workers from the risks related to exposure to asbestos at work. Official Journal L97 of 15.4.2003, 48.
Dodson RF, Atkinson MA, Levin JL (2003) Asbestos fiber length as related to potential pathogenicity: a critical review. Am J Ind Med 44:291–297
Dumortier P, Rey F, Viallant JR, Broucke I, Boutin C, De Vuyst P (2002) Chrysotile and tremolite asbestos fibres in the lungs and parietal pleura of Corsican goats. Occup Environ Med 59:643–646
Englert JM, Kliment CR, Oury TD (2014) Experimental models of asbestos-related diseases. In: T.D. Oury, T.A. Sporn, V.L. Roggli (eds), Pathology of asbestos-associated diseases 215–251.
Fornero E, Belluso E, Capella S, Bellis D (2009) Environmental exposure to asbestos and other inorganic fibres using animal lung model. Sci Total Environ 407:1010–1018
Gianfagna A, Ballirano P, Bellatreccia F, Bruni B, Paoletti L, Oberti R (2003) Characterization of amphibole fibres linked to mesothelioma in the area of Biancavilla, Eastern Sicily, Italy. Mineral Mag 67:1221–1229
Groppo C, Compagnoni R (2007) Metamorphic veins from the serpentinites of the Piemonte Zone, western Alps, Italy: a review. Periodico di Mineralogia 76:127–153
Hawthorne FC, Oberti R, Harlow GE, Maresch WV, Martin RF, Schumacher JC, Welch MD (2012) Nomenclature of the amphibole supergroup. Am Mineral 97(11–12):2031–2048
Hillerdal G (1999) Mesothelioma: cases associated with non-occupational and low dose exposure. Occup Environ Med 56:505–513
Hobbs C (2003) Fiber durability and biopersistence—assessment and role in asbestos toxicology Asbestos Mechanisms of Toxicity Workshop, June 12–13, 2003 Chicago, Illinois
Kakooei H, Yunesian M, Marioryad AK (2009) Assessment of airborne asbestos fiber concentrations in urban area of Tehran, Iran. Air Quality Atmosphere & Health 2:39–45
Leake BE (1978) Nomenclature of amphiboles. Can Mineral 16:501–520
Leake BE, Woolley AR, Arps CES, Birch WD, Gilbert MC, Grice JD, Hawthorne FC, Kato A, Kisch HJ, Krivovichev VG, Linthout K, Laird J, Mandarino JA, Maresch WV, Nickel EH, Rock NMS, Schumacher JC, Smith DC, Stephenson NCN, Ungaretti L, Whittaker EJW, Youzhi G (1997) Nomenclature of amphiboles: report of the subcommittee on amphiboles of the international mineralogical association, commission on new minerals and mineral names. Can Mineral 35:219–246
Leake BE, Woolley AR, Birch WD, Burke EAJ, Ferraris G, Grice JD, Hawthorne FC, Kisch HJ, Krivovichev VG, Schumacher JC, Stephenson NCN, Whittaker EJW (2003) Nomenclature of amphiboles: additions and revisions to the International Mineralogical Association’s amphibole nomenclature. Can Mineral 41:1355–1370
Luce D, Buge I, Goldberg P, Goldberg M, Salomon C, Billon-Galland MA, Nicolau J, Quenel P, Fevotte J, Brochard P (2000) Environmental exposure to tremolite and respiratory cancer in New Caledonia: a case-control study. Am J Epidemiol 151:259–265
Martin RF (2007) Amphiboles in the igneous environment. In: J.J. Rosso (ED), Amphiboles: crystal chemistry, occurrence, and health issues. Rev Mineral Geochem 67:323–357
McClellan RO (2012) Role of science and judgment in setting national ambient air quality standards: how low is low enough? Air Quality, Atmosphere & Health 5:243–258
Meerker GP, Bern AM, Brownfield IK, Lowers HA, Sutley SJ, Hoefen TM, Vance JS (2003) The composition and mineralogy of amphiboles from the Rainy Creek Complex, near Libby, Montana. Am Mineral 88:1955–1969
NRC (National Research Council), Committee on Animals as Monitors of Environmental Health Hazards (1991) Animals as sentinels of environmental health hazards. National Academy Press, Washington, DC, p. 160
Piccoli GC, Maletto G, Bosio P, Lombardo B (2007) Minerali del Piemonte e della Valle d'Aosta. Associazione Amici del Museo “F. Eusebio” Alba, (Cuneo) p 607
Regione Piemonte (2005) Explanatory notes to agricultural and forest landscape map. http://www.regione.piemonte.it/agri/area_tecnico_scientifica/suoli/paesaggi/dwd/note.pdf"\t blank
Regione Piemonte (2012) http://arpa.piemonte.gov.it/approfondimenti/temi-ambientali/geologia-edissesto/bancadati/cartografia-geologia
Regione Piemonte (2013) http://www.regione.piemonte.it/agri/psr2014_20/dwd/vas/BozzaPSR_1305_2013.pdf)
Roggli VL (1990) Human disease consequences of fiber exposures: a review of human lung pathology and fiber burden data. Environ Health Persp 88:295–303
Roggli VL (2015) The so-called short-fiber controversy: literature review and critical analysis. Arch Pathol Lab Med 139:1052–1057
Roggli VL, Vollmer RT, Butnor KJ, Sporin TA (2002) Tremolite and mesothelioma. Ann Occup Hyg 46(5):447–453
Schumacher JC (2007) Metamorphic amphiboles: composition and coexistence. In: J.J. Rosso (Ed), Amphiboles: crystal chemistry, occurrence, and health issues. Rev Mineral Geochem 67:359–416.
Tossavainen A (1997) Asbestos, asbestosis and cancer. The Helsinki criteria for diagnosis and attribution. Consensus Report Scand. J Work Environ Health 23:211–216
WHO (1997) Asbestos and other natural mineral fibres. International Programme for
Yazicioglu S, Ilçaito R, Balci K, Sayli BS (1980) Plural calcifications, pleural mesothelioma and bronchial cancers caused by tremolite dusts. Torax 35:564–569
Acknowledgments
This study was financially supported by Istituto Nazionale per la Ricerca Scientifica e Tecnologica sulla Montagna (INRM), Assessorato all’Ambiente della Regione Piemonte, and Ricerca Sanitaria Finalizzata ASL TO4 (2006).
The authors wish to thank Dr. Giordano for map preparation.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Capella, S., Fornero, E., Bellis, D. et al. Analysis of respired amphibole fibers (asbestos and non-asbestos classified): discrimination between natural and anthropogenic sources using sentinel animals. Air Qual Atmos Health 10, 533–542 (2017). https://doi.org/10.1007/s11869-016-0448-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11869-016-0448-6