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Synthesis, Characterization, In Vitro Bioactivity and Biocompatibility Evaluation of Hydroxyapatite/Bredigite (Ca7MgSi4O16) Composite Nanoparticles

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Abstract

Silicate-based bioceramics have been found to possess excellent apatite-forming ability, and they can stimulate cell proliferation and osteogenic differentiation. In this study, bredigite (Ca7MgSi4O16) nanoparticles were synthesized and incorporated into a hydroxyapatite (HA)-based matrix to produce composite nanoparticles with improved bioactivity and biocompatibility. HA/bredigite nanoparticles containing 25% and 50% bredigite were synthesized by using the sol–gel method. X-ray diffraction, transmission electron microscopy, scanning electron microscopy, energy-dispersive x-ray spectroscopy, and Fourier transform infrared techniques were used to study the phase structure, morphology, and structural properties of prepared nanoparticles. Results indicated that HA/bredigite nanoparticles with an average particle size of less than 50 nm and homogeneous distribution of bredigite were successfully synthesized. Obtained results also revealed that the presence of bredigite led to a small increase in HA lattice parameters and to a decrease in the agglomeration of composite nanoparticles. The in vitro bioactivity studies performed in the simulated body fluid showed that composite nanoparticles had higher apatite-forming ability than pure HA. The results of a cell proliferation assay revealed that the proliferation of mesenchymal stem cells in the extract of HA/bredigite was significantly higher than those in the extract of the initial HA and control group after 72 h. As the properties of HA/bredigite nanoparticles were highly improved, compared with pure HA, it is concluded that these composite nanoparticles could potentially be good candidates for use as effective bioactive materials in bone regeneration applications.

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Acknowledgements

The authors express their sincere gratitude to the Isfahan University of Technology for financial support. This project was also supported by the Department of Tissue Engineering and Regenerative Medicine of Iran University of Medical Sciences.

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Authors and Affiliations

  1. Biomaterials Research Group, Department of Materials Engineering, Isfahan University of Technology, 8415683111, Isfahan, Iran

    Monireh Kouhi, Morteza Shamanian & Mohammadhossein Fathi

  2. Dental Materials Research Center, Isfahan University of Medical Sciences, 8174673461, Isfahan, Iran

    Mohammadhossein Fathi

  3. Cellular and Molecular Research Center, Iran University of Medical Sciences, 1445613131, Tehran, Iran

    Ali Samadikuchaksaraei & Ahmad Mehdipour

  4. Department of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, 1445613131, Tehran, Iran

    Ali Samadikuchaksaraei & Ahmad Mehdipour

  5. Department of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Science, 1445613131, Tehran, Iran

    Ali Samadikuchaksaraei

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  1. Monireh Kouhi
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  2. Morteza Shamanian
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  3. Mohammadhossein Fathi
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  4. Ali Samadikuchaksaraei
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  5. Ahmad Mehdipour
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Correspondence to Monireh Kouhi.

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Kouhi, M., Shamanian, M., Fathi, M. et al. Synthesis, Characterization, In Vitro Bioactivity and Biocompatibility Evaluation of Hydroxyapatite/Bredigite (Ca7MgSi4O16) Composite Nanoparticles. JOM 68, 1061–1070 (2016). https://doi.org/10.1007/s11837-016-1815-3

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  • DOI: https://doi.org/10.1007/s11837-016-1815-3

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