Abstract
Jojoba (Simmondsia chinensis), is a medicinal and oil-yielding, multi-purpose species of the family Simmondsiaceae. The most valuable product of jojoba seed is the liquid wax or jojoba oil which is used extensively in the cosmetic and bio-fuel industry. Propagation of jojoba is possible using conventional methods, but it is time consuming and cumbersome owing to long rotation periods, male-biased population, and long flowering and seed set time. The development of an efficient regeneration system is a prerequisite for a number of biotechnological interventions for the improvement of jojoba, such as genetic transformation, production of useful metabolites in vitro, etc. During the past decade, therefore, several attempts have been made for in vitro propagation of jojoba. Organogenesis has been achieved in this species from mature as well as juvenile explants. Present communication reports an overview of the in vitro regeneration of jojoba via organogenesis and somatic embryogenesis. Factors affecting organogenesis as well as production of synthetic seeds using shoot tips and axillary buds have also been discussed; however, efforts need to be made to develop an efficient genetic transformation system in jojoba. The purpose of this review is to focus upon the current information on in vitro propagation and biotechnological advances made in jojoba.
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References
Agarwal M, Shrivastava N, Padh H (2011) Development of sex-linked AFLP markers in Simmondsia chinensis. Plant Breed 130:114–116
Agrawal V, Prakash S, Izhar S (1999) Differential hormonal requirements for clonal propagation of male and female jojoba plants. In: Altman A, Ziv M (eds) Plant biotechnology and in vitro biology in the 21st century. Proceedings of the 9th international congress of the international association of plant tissue culture biotechnology, Jerusalem, Israel, 14–19 June 1998, pp 25–28
Agrawal V, Prakash S, Gupta SC (2002) Effective protocol for in vitro shoot production through nodal explants of Simmondsia chinensis. Biol Plant 45:449–453
Agrawal V, Sharma K, Sarika G, Kumar R, Prasad M (2007) ISSR marker-assisted selection of male and female plants in a promising dioecious crop: jojoba (Simmondsia chinensis). Plant Biotech Rep 2:239–243
Amarger V, Mercier L (1995) Molecular analysis of RAPD DNA based markers: their potential use for the detection of genetic variability in jojoba (Simmondsia chinensis L Schneider). Biochimie 77:931–936
Amin MN, Jaiswal VS (1984) In vitro propagation of guava (Psidium guajava L.): effects of sucrose, agar and pH on growth and proliferation of shoots. Bangladesh J Bot 18:1–8
Amin MN, Jaiswal VS (1987) Rapid clonal propagation of guava through in vitro shoot proliferation on nodal explants of mature trees. Plant Cell Tissue Organ Cult 9:235–243
Anonymous (1975) Under-exploited tropical plants with promising economic value. National Academy of Sciences. Washington, DC
Apostolo NM, Llorente B (2000) Anatomy of normal and hyperhydric leaves and shoots of in vitro grown Simmondsia chinesis (Link) Schn. In Vitro Cell Dev Biol Plant 36:414–418
Apostolo NM, Brutti C, Ferrarotti SA, Llorente BE, Krymkiewicz NL (2001) Stimulation of root development with cyclodextrins on jojoba shoots in vitro. In Vitro Cell Dev Biol Plant 37:414–418
Bajaj YPS (1986) Biotechnology of tree improvement for rapid propagation and biomass energy production. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 1. Springer, Berlin, pp 1–23
Bashir MA, Rashid H, Anjum MA (2007a) In vitro shoot multiplication of six promising strains of jojoba (Simmondsia chinensis). Biotechnology 6:309–315
Bashir MA, Anjum MA, Rashid H (2007b) In vitro root formation in micropropagated shoots of jojoba (Simmondsia chinensis). Biotechnology 6:465–472
Bashir MA, Muhammad AA, Hamid R (2008) In vitro propagation of some promising genotypes of jojoba (Simmondsia chinensis). Afr J Biotech 7:3878–3886
Benzioni A (1995) Jojoba domestication and commercialization in Israel. Hortic Rev 17:234–266
Benzioni A, Mills D, Wenkart S, Zhou Y (2003) Effects of ventilation on the performance of jojoba (Simmondsia chinensis) clones: multiplication stage. Acta Hortic 616:135–138
Bhardwaj M, Uppal S, Jain S, Kharb P, Dhillon R, Jain RK (2010) Comparative assessment of ISSR and RAPD marker assays for genetic diversity analysis in jojoba [Simmondsia chinensis (Link) Schneider]. J Plant Biochem Biotechnol 19:255–258
Bhojwani SS, Razdan MK (1996) Plant tissue culture: theory and practice. Elsevier, Amsterdam
Birnbaum E, Matias S, Wenkart S (1984) Vegatative propagation of jojoba by tissue culture. In: Wisniak J, Zabicky J (eds) Proceedings of the sixth international conference on jojoba and its uses. Ben Gurion University, Beer-Sheva, pp 233–241
Chaturvedi HC, Sharma M (1989) In vitro production of cloned plants of jojoba (Simmondsia chinensis (Link) Schneider) through shoot proliferation in long term culture. Plant Sci 63:199–207
Christianson ML (1987) Causal events in morphogenesis. In: Green CE, Somers DA, Hackett WP, Biesboer DD (eds) Plant tissue and cell culture. Liss, New York, pp 44–55
Dewan A, Nanda K, Gupta SC (1992) In vitro propagation of Acacia nilotica subsp Indian breman via cotyledonary nodes. Plant Cell Rep 12:18–21
Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15
Driver JA, Kuniyuki AH (1984) In vitro propagation of paradox walnut rootstock. Hortic Sci 19:507–509
Elhag H, El-Olemy MM, Mossa JS, Tag-El-Din SS, Al-Zoghet MF, Al-Alsheikh AMA (1998) In vitro propagation of jojoba. In: Program abstract of the annual conference on new crops and new uses: biodiversity and sustainability, Phoenix, AZ
Engleman F, Engles J, Dullo E (2003) The development of complementary strategies for the conservation of plant genetic resources using in vitro and cryopreservation methods. In: Chaudhury R, Pandey R, Malik SK, Bhag Mal (eds) In vitro conservation and cryopreservation of tropical fruit species. IPGRI, NBPGR, New Delhi, pp 37–48
Gaber A, El-Maraghy HM, Aly MA, Rashed NA, El-Din AY (2007) Induction of somatic embryogenesis and DNA fingerprinting of jojoba. Arab J Biotechnol 10:341–354
Gao HD, Cao B (2001) Study on technology of tissue culture of Simmondsia chinensis (Link) Schneider (in Chinese). J Jiangsu For Sci Technol 28:12–14
Gautheret RJ (1955) Nutrition of plant cultures. Annu Rev Plant Physiol 6:435–484
Giri C, Shyamkumar B, Anjaneyulu C (2004) Progress in tissue culture, genetic transformation and applications of biotechnology to trees: an overview. Trees Struct Funct 18:115–135
Gulati A, Jaiwal PK (1996) Micropropagation of Dalbergia sissoo from nodal explant of mature trees. Biol Plant 38:169–175
Gupta AK (2001) Jojoba seed and oil from Indian locations for quality. In: Proceeding of national seminar on production, marketing and processing of jojoba (Simmondsia chinensis), Jaipur, India, (abstr.), pp 40–41
Haberlandt G (1902) Culturversuche mit isolierten. Pflanzenzellen Sitzungsber Akad Wiss Wien Math Nat Cl III Abt, vol 1, pp 69–91
Hamama L, Baaziz M, Letouze R (2001) Somatic embryogenesis and plant regeneration from leaf tissue of jojoba. Plant Cell Tissue Organ Cult 65:109–113
Harris PJC, Moore THM, Puddephat IJ (1989) Micropropagation of Sesbania sesban. Nitrogen Fixing Tree Res Rep 7:123–125
Harsh LN, Tiwari JC, Bohra MD, Tripathi D (2001) Standardization of agronomic practices of jojoba cultivation in arid regions. In: Proceeding of national seminar on production, marketing and processing of jojoba (Simmondsia chinensis), Jaipur, India, (abstr.), pp 31–32
Hassan NS (2003) In vitro propagation of jojoba (Simmondsia chinensis L) through alginate-encapsulated shoot apical and axillary buds. Int J Agric Biol 5:513–516
Hosseini FS, Hassani HS, Arvin MJ, Baghizadeh A, Nejad GM (2011) Sex determination of jojoba (Simmondsia chinensis cv. Arizona) by random amplified polymorphic DNA (RAPD) molecular markers. Afr J Biotechnol 10:470–474
Ince AG, Karaca MA, Onus AN (2010) A reliable gender diagnostic PCR assay for jojoba (Simmondsia chinensis (Link) Schneider). Genet Resour Crop Evol 57:773–779
Jacoboni A, Standardi A (1987) Tissue culture of jojoba (Simmondsia chinensis Link). Acta Hortic 212:557–560
John A, Murray BW (1981) Micropropagation of sitka spruce (Picea sitchensis). In: Colloque international surla culture in vitro des essences, Fountainebleau, AFOCEL, pp 65–70
Kacker NL, Joshi SP, Singh M, Solanki KR (1993) In vitro regeneration of female plants of Simmondsia chinensis (Link) Schneider (Jojoba) using coppice shoots. Ann Arid Zone 32:175–177
Karp A (1994) Origin, causes and uses of variation in plant tissue cultures. In: Vasil IK, Thorpe TA (eds) Plant cell and tissue culture. Kluwer, Dordrecht, pp 139–150
Keathley DE (1984) Micropropagation of mature spruce. In: Karnosky H, Keathly DE (eds) International symposium on recent advances in forest biotechnology. Traverse City, Michigan, pp 58–63
Kim SW, Liu JR (1999) Somatic embryogenesis and plant regeneration in zygotic embryo cultures of balloon flower. Plant Cell Tissue Organ Cult 58:227–230
Komamine A, Murata N, Nomura K (2005) Mechanisms of somatic embryogenesis in carrot suspension cultures-morphology, physiology, biochemistry, and molecular biology. In Vitro Cell Dev Biol Plant 41:6–10
Krishna H, Singh SK (2007) Biotechnological advances in mango (Mangifera indica L.) and their future implication in crop improvement—a review. Biotechnol Adv 25:223–243
Kumar S, Singh N, Mangal M (2009a) Biochemical changes during shoot differentiation in callus culture of jojoba (Simmondsia chinensis). J Plant Biol 36:11–16
Kumar S, Singh N, Mangal M (2009b) Micropropagation of Simmondsia chinensis (Link) Schneider through enhanced axillary branching from nodal segments. J Plant Biol 36:75–81
Kumar N, Modi AR, Singh AS, Gajera BB, Patel AR, Patel MP, Subhash N (2010a) Assessment of genetic fidelity of micropropagated date palm (Phoenix dactylifera L.) plants by RAPD & ISSR markers assay. Physiol Mol Biol Plants 16:207–213
Kumar S, Rai MK, Singh N, Mangal M (2010b) Alginate-encapsulation of shoot tips of jojoba [Simmondsia chinensis (Link) Schneider] for germplasm exchange and distribution. Physiol Mol Biol Plants 16:379–382
Kumar S, Mangal M, Dhawan AK, Singh N (2011) Assessment of genetic fidelity of micropropagated plants of Simmondsia chinensis (Link) Schneider using RAPD and ISSR markers. Acta Physiol Plant. doi:10.1007/s11738-011-0767-z
Lee CW (1988) Application of plant biotechnology for clonal propagation and yield enhancement in jojoba. In: Baldwin AR (ed) Proceeding of the seventh international conference on jojoba and its uses. Illinois, USA, pp 102–111
Lee CW, Palzkill DA (1984) Propagation of jojoba by single node cutting. Hortic Sci 19:841–842
Lee CW, Thomas JC (1985) Jojoba embryo culture and oil production. Hortic Sci 20:762–764
Litz RE, Gray DJ (1992) Organogenesis and somatic embryogenesis. In: Hammerschlag FA, Litz RE (eds) Biotechnology of perennial fruit crops. CAB International, Wallingford, pp 3–34
Llorente B, Apostolo NM (1998) Effect of different growth regulators and genotype on in vitro propagation of jojoba. N Z J Crop Hortic Sci 26:55–62
Low CB, Hackett WP (1981) Vegetative propagation of jojoba. Calif Agric 35:12–13
Malik SK, Chaudhury R, Kalia RK (2005) Rapid in vitro multiplication and conservation of Garcinia indica: a tropical medicinal tree species. Sci Hortic 106:539–553
Mandani A, Lee CW, Hogan L (1978) In vitro propagation of Simmondsia chinensis via shoot tip culture. Hortic Sci 13:35–37
McCown B, Amos R (1979) Initial trials with commercial micropropagation of birch selections. Proc Int Plant Prop Soc 29:387–393
Meyghani H, Ghazvini RF, Hamidoghli Y (2005) Micropropagation from stem segments of salt tolerant jojoba seedlings. J Korean Soc Hortic Sci 46:83–187
Mills D, Benzioni A (1992) The effect of NaCl salinity on growth and development of jojoba clones: II. Nodal segments grown in vitro. J Plant Physiol 139:737–741
Mills D, Wenkart S, Benzioni A (1997) Micropropagation of Simmondsia chinensis (Jojoba). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 40. High-tech and micropropagation VI. Springer, Berlin, pp 370–393
Mills D, Yanqing Z, Benzioni A (2004) Improvement of jojoba shoot multiplication in vitro by ventilation. In Vitro Cell Dev Biol Plant 40:396–402
Mohammed AM, Aly Essam A, Amer Wasef A, Al-Zayadneh AE, Negm E (2008) Growth regulators influence the fatty acid profiles of in vitro induced jojoba somatic embryos. Plant Cell Tissue Organ Cult 93:107–114
Murashige T (1974) Plant propagation through tissue cultures. Annu Rev Plant Physiol 25:135–166
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Naik SK, Chand PK (2006) Nutrient-alginate encapsulation of in vitro nodal segments of pomegranate (Punica granatum L.) for germplasm distribution and exchange. Sci Hortic 108:247–252
Nandwani D, Ramawat KG (1993) In vitro plantlet formation through juvenile and mature explants in Prosopis cineraria. Indian J Exp Biol 3:156–160
Nehra NS, Kartha KK (1994) Meristem and shoot tip culture: requirement and application. In: Vasil IK, Thorpe TA (eds) Plant cell and tissue culture. Kluwer, Dordrecht, pp 37–70
Pati PK, Rath SP, Sharma M, Sood A, Ahuja PS (2006) In vitro propagation of rose—a review. Biotechnol Adv 24:94–114
Phillips RL, Kaeppler SM, Olhoft P (1994) Genetic instability of plant tissue cultures: breakdown of normal controls. Proc Natl Acad Sci USA 91:5222–5226
Philomina NS, Rao JVS (1999) Multiple shoots production from seed culture of soap nut (Sapindus mukorossi Gaertn.). Phytomorphology 49:419–423
Prakash S, Agrawal V, Gupta SC (2003) Influence of some adjuvants on in vitro clonal propagation of male and female jojoba plants. In Vitro Cell Dev Biol Plant 39:217–222
Raghava Swamy BV, Himabindu K, Sita GL (1992) In vitro micropropagation of elite rose wood (Dalbergia latifolia Roxb.). Plant Cell Rep 11:126–131
Rai MK, Asthana P, Singh SK, Jaiswal VS, Jaiswal U (2009) The encapsulation technology in fruit plants—a review. Biotechnol Adv 27:671–679
Rai MK, Asthana P, Jaiswal VS, Jaiswal U (2010) Biotechnological advances in guava (Psidium guajava L.): recent development and prospects for future research. Trees 24:1–12
Rao PS, Suprasanna P, Ganapathi TR (1996) Plant biotechnology and agriculture prospects for improvement and increasing plant productivity. Sci Cult 62:185–191
Reddy MP, Chikara J (2010) Biotechnology advances in jojoba (Simmondsia chinensis). In: Ramawat KG (ed) Desert plants. Springer, Berlin, pp 407–422
Roja G, Rao PS (1998) Biotechnological investigation in medicinal plants for the production of secondary metabolites. In: Khan I, Khanum A (eds) Role of biotechnology in medicinal and aromatic plants. Ukaaj, Hyderabad, pp 95–125
Rost TL, Hinchee MAW (1980) Preliminary report of the production of callus, organogenesis and regeneration of jojoba (Simmondsia chinensis (Link) Schneider) in tissue culture. J Hortic Sci 55:299–305
Roussos PA, Tolia-Marioli A, Pontikis CA, Kotsias D (1999) Rapid multiplication of jojoba seedling by in vitro culture. Plant Cell Tissue Organ Cult 57:133–137
Rout GR, Samantaray S, Das P (2000) In vitro manipulation and propagation of medicinal plants. Biotechnol Adv 18:91–120
Rumary C, Thorpe TA (1984) Plantlet formation in black and white spruce. Can J Res 14:10–16
Saiprasad GVS (2001) Artificial seeds and their application. Resonance 39–47
Sardana J, Batra A (1998) In vitro regeneration of jojoba (Simmondsia chinensis): a plant of high potential. Adv Plant Sci 11:143–146
Scaramuzzi F, D’Ambrosio A (1988) Organogenesis and propagation in vitro of Simmondsia chinensis (Link) Schn (jojoba) from vegetative fragments. Acta Hortic 227:411–413
Schenk RU, Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50:199–204
Shahzad A, Siddiqui SA (2001) Micropropagation of Melia azedarach L. Phytomorphology 51:151–154
Sharma K, Agrawal V, Sarika G, Kumar R, Prasad M (2008) ISSR marker-assisted selection of male and female plants in a promising dioecious crop: jojoba (Simmondsia chinensis). Plant Biotechnol Rep 2:239–243
Sharma R, Chowdhury V, Jain S, Jain RK (2009) A comparative study of genetic relationships among and within male and female genotypes of dioecious jojoba (Simmondsia chinensis L. Schneider) using RAPD and ISSR markers. Asian J Hortic 4:184–193
Singh M, Jaiswal U, Jaiswal VS (2004) In vitro regeneration and improvement in tropical fruit trees: an assessment. In: Srivastava PS, Narula A, Srivastava S (eds) Plant biotechnology and molecular markers. Anamanya, New Delhi, pp 228–243
Singh A, Reddy MP, Patolia JS (2008) An improved protocol for micropropagation of elite genotype of Simmondsia chinensis (Link) Schneider. Biol Plant 52:538–542
Singh SR, Singh R, Dhawan AK (2009) Biochemical changes related to shoot differentiation in callus cultures of Tylophora indica wight and arn. J Indian Bot Soc 88:49–53
Smith DL, Krikorian AD (1991) Growth and maintenance of an embryogenic cell culture of daylily (Hemerocallis) on hormone free medium. Ann Bot 67:443–447
Thakur M, Sharma DR, Kanwar K (2001) Mass micropropagation of Alnus nepalensis D. Don. Phytomorphology 51:123–127
Thomas TD (2008) The role of activated charcoal in plant tissue culture. Biotechnol Adv 26:618–631
Tyagi RK, Prakash S (2004) Genotype and sex specific protocols for in vitro micropropagation and medium term conservation of jojoba. Biol Plant 48:119–123
Undersander DJ, Oelke EA, Kaminski AR, Doll JD, Putnam DH, Combs SM, Hanson CV (1990) Jojoba. http://www.hort.purdue.edu/newcrop/afcm/jojoba.html
Wang YC, Janick J (1986a) Somatic embryogenesis in jojoba. J Am Soc Hortic Sci 111:281–287
Wang YC, Janick J (1986b) In vitro production of jojoba liquid wax by zygotic and somatic embryos. J Am Soc Hortic Sci 111:798–806
Zimmerman JL (1993) Somatic embryogenesis: a model of early development in higher plants. Plant Cell 5:1411–1423
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Kumar, S., Mangal, M., Dhawan, A.K. et al. Biotechnological advances in jojoba [Simmondsia chinensis (Link) Schneider]: recent developments and prospects for further research. Plant Biotechnol Rep 6, 97–106 (2012). https://doi.org/10.1007/s11816-011-0211-2
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DOI: https://doi.org/10.1007/s11816-011-0211-2