Abstract
The glycogen content and the activities of two key enzymes in glycogen metabolism, glycogen phosphorylase and glycogen synthetase, in the gonad of diploid and triploid Pacific oysters (Crassostrea gigas) were compared during maturation. The glycogen content in the gonad of diploids decreased with gametogenesis (by 85.7%), but the glycogen content in the gonad of triploids did not vary significantly. Activity of glycogen phosphorylase (GP) in the gonad of diploids decreased with gametogenesis (by 55.5%), while GP activity of triploids did not vary significantly during maturation. Activity of glycogen synthetase (GS) in the gonad of diploids increased slightly with gametogenesis, reaching a peak in June. Activity of GS declined sharply from June to July, which might be due to gonad spawning. GS activity of triploid oysters in spawning time (July and August) was significantly higher than that in other months, which might be explained with a ‘compensating’ mechanism for the higher glycogen content in triploids.
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Akashige, S., and T. Fushimi, 1992. Growth, survival, and glycogen content of triploid Pacific oyster (Crassostrea gigas Thunberg) in the waters of Hiroshima, Japan. Nippon Suisan Gakk., 58: 1063–1071.
Allen, S. K. Jr., B. Eudeline, Y. Naciri, and A. Gerard, 1994. Estimating the ecological impact of triploid Crassostrea gigas: what is the meaning of sterility? Final Report. Contract No. 94/55 66 402. IFREMER-Rutgers University, 33 pp.
Allen, S. K. Jr., and S. L. Downing, 1986. Performance of triploid Pacific oysters (Crassostrea gigas Thunberg). I. Survival, growth, glycogen content, and sexual maturation in yearlings. J. Exp. Mar. Biol. Ecol., 102: 197–208.
Allen, S. K. Jr., and S. L. Downing, 1990. Performance of triploid Pacific oyster, Crassostrea gigas: Gametogenesis. Can. J. Fish. Aquat. Sci., 47: 1213–1222.
Allen, S. K. Jr., M. S. Shpigel, S. Utting, and B. Spencer, 1994. Incidental production of tetraploid Manila clams, Tapes philippinarum. Aquaculture, 128: 13–19.
Bayne, B. L., A. Bubel, P. A. Gabbott, D. R. Livingstone, D. M. Lowe, et al., 1982. Glycogen utilization and gametogenesis in Mvtilus edulis L. Mar. Biol. Lett., 3: 89–105.
Berthelin, C., K. Kellner, and M. Mathieu, 2000. Storage metabolism in the Pacific oyster (Crassostrea gigas) in relation to summer mortalities and reproductive cycle (West Coast of France). Comp. Biochem. Physiol. B, 125: 359–369.
Brokordt, K. B., J. H. Himmelman, and H. E. Guderley, 2000. Effect of reproduction on escape responses and muscle metabolic capacities in the scallop Chalmys islandica Muller 1776. J. Exp. Mar. Biol. Ecol., 251: 205–225.
Cherian, S., and G. Philip, 1992. Comparative studies on the properties of glycogen phosphorylases from the foot muscles of two benthic bivalves, Sunetta scripta and Villorita cyprenoides, having different habitats. Indian J. Biochem. Biophys., 29: 87–92.
Gabbott, P. A., and M. A. Whittle, 1986. Glycogen synthetase in the sea mussel Mytilus edulis. 1–2. Seasonal changes in glycogen content and glycogen synthetase activity in the mantle tissue. Comp. Biochem. Physiol. B, 83: 197–207.
Gabbott, P. A., 1975. Storage cycles in marine bivalve mollusks: a hypothesis concerning the relationship between glycogen metabolism and gametogenesis. In: Proceedings of the 9th European Marine Biology Symposium. Barnes, H. ed., Abeerden University Press, Abeerden, 191–211.
Greenway, S. C., and K. B. Storey, 1999. The effect of prolonged anoxia on enzyme activities in oysters (Crassostrea virginica) at different seasons. J. Exp. Mar. Biol. Ecol., 242: 259–272.
Hata, K., M. Hata, and K. Matsuda, 1993. Purification and properties of glycogen phosphorylase from the adductor muscle of the oyster, Crassostrea gigas. Comp. Biochem. Physiol. B, 105: 481–486.
Hata, K., I. Yokoyama, M. Suda, M. Hata, and K. Matsuda, 1987. Purification and properties of glycogen phosphorylase from the adductor muscle of the scallop, Patinopecten yessoensis. Comp. Biochem. Physiol. B, 87: 747–753.
Li, Q., M. Osada, and K. Mori, 2000. Seasonal biochemical variations in Pacific oyster gonadal tissue during sexual maturation. Fish. Sci., 66: 502–508.
Mathieu, M., and P. Lubet, 1993. Storage tissue metabolism and reproduction in marine bivalves — a brief review. Invertebr. Reprod. Dev., 23: 123–129.
Ruiz-Verdugo, C. A., I. S. Racotta, and A. M. Ibarra, 2001. Comparative biochemical composition in gonad and adductor muscle of triploid and diploid catarina scallop Argopecten ventricosus Sowerby II, 1842. J. Exp. Mar. Biol. Ecol., 259: 155–170.
San Juan Serrano, F., M. Fernandez-Gonzalez, J. L. Sanchez-Lopez, and L. O. Garcia-Martin, 1991. Purification and molecular properties of glycogen phosphorylase b from mantle tissue of mussel, Mytilus galloprovincialis. Comp. Biochem. Physiol. B, 98: 33–39.
Swift, M. L., K. O. Akosah, T. P. Thomas, and C. L. Humphrey, 1988. Characteristics of glycogen synthase activity in the digestive diverticula of the oyster, Crassostrea virginica Gmelin. J. Shellfish Res., 7: 177–178.
Tabarini, C. L., 1984. Induced triploidy in the bay scallop, Argopecten irradians and its effect on growth and gametogenesis. Aquaculture, 42: 151–160.
Utting, S. D., P. F. Millican, and I. Laing, 1996. The breeding potential. and biochemical composition of triploid Manila clams, Tapes philippinarum Adams and Reeve. Aquacult. Res., 27: 573–580.
Vardanis, A., 1990. Fractionation of particulate glycogen and bound enzymes using high-performance liquid chromatography. Anal. Biochem., 187: 115–119.
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Kong, L., Wang, Z., Yu, R. et al. Seasonal variation of the glycogen enzyme activity in diploid and triploid Pacific oyster gonad during sexual maturation. J Ocean Univ. China 6, 383–386 (2007). https://doi.org/10.1007/s11802-007-0383-0
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DOI: https://doi.org/10.1007/s11802-007-0383-0