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Phytotoxicity in seven higher plant species exposed to di-n-butyl phthalate or bis (2-ethylhexyl) phthalate

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Abstract

We investigated phytotoxicity in seven plant species exposed to a range of concentrations (0–500 mg·kg−1 soil) of di-n-butyl phthalate (DnBP) or bis (2-ethylhexyl) phthalate (DEHP), two representative phthalate esters (PAEs) nominated by USEPA as priority pollutants and known environmental estrogens. We studied seed germination, root elongation, seedling growth, biomass (fresh weight, FW) and malondialdehyde (MDA) content of shoots and roots of wheat (Triticum aestivum L.), alfalfa (Medicago sativa L.), perennial ryegrass (Lolium perenne), radish (Raphanus sativus L.), cucumber (Cucumis sativus L.), oat (Avena sativa) and onion (Allium cepa L.), together with monitoring of plant pigment content (chlorophyll a, b and carotinoids) in alfalfa, radish and onion shoots. Root elongation, seedling growth and biomass of the test species were generally inhibited by DnBP but not by DEHP, indicating a lower level of phytotoxicity of DEHP than of DnBP. MDA contents of four species were promoted by PAE exposure, but not in alfalfa, ryegrass or onion shoots, indicating lower sensitivity of these three species to PAE pollutants. Plant pigment contents were clearly affected under the stress of both pollutants, implying the potential damage to the photosynthetic system of test plants, mainly by decreasing the content of chlorophyll a and b. Results of DnBP and DEHP phytotoxicity to the primary growth of test plants has provided information for the assessment of their environmental risk in the soil and also forms a basis for the further analysis of their toxic effects over the whole growth period of different plant species.

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Authors and Affiliations

  1. Key Laboratory of Soil Environment and Pollution Remediation of Chinese Academy of Sciences, Institute of Soil Science, Nanjing, 210008, China

    Tingting Ma, Ying Teng & Yongming Luo

  2. Graduate University of Chinese Academy of Sciences, Beijing, 100049, China

    Tingting Ma, Ying Teng & Yongming Luo

  3. Agri-Environment Branch, Agri-Food and Biosciences Institute, Newforge Lane, Belfast, BT9 5PX, UK

    Peter Christie

  4. Yantai Institute of Costal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China

    Yongming Luo

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  1. Tingting Ma
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  2. Ying Teng
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  4. Yongming Luo
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Correspondence to Yongming Luo.

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Ma, T., Teng, Y., Christie, P. et al. Phytotoxicity in seven higher plant species exposed to di-n-butyl phthalate or bis (2-ethylhexyl) phthalate. Front. Environ. Sci. Eng. 9, 259–268 (2015). https://doi.org/10.1007/s11783-014-0652-2

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