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Antioxidants in Fish Oil Production for Improved Quality

  • Original Paper
  • Published:
Journal of the American Oil Chemists' Society

Abstract

There is an increasing demand for fish oil containing omega-3. By-products (e.g., heads, tails, belly flaps, backbones, and viscera) from fish fillet production are currently utilized for production of fish oil and fish meal for feed but can be a valuable source of omega-3 for human consumption. However, the quality of some oil is not good enough for human consumption due to oxidation during processing. The aim of this work was to decrease oxidation during oil production by adding antioxidants to herring by-products early in the production process. Of several antioxidants the most potent were selected by a fast screening test. Butylated hydroxytoluene, propyl gallate and citric acid were further evaluated on a labaroatory scale and pilot plant production of herring oil. The selected antioxidants resulted in lower total oxidation during processing [up to 71% reduction in TOTOX (2 times the peroxide value plus the anisidine value)]. In addition, higher oil stability was achieved by adding antioxidants during processing (improved stability up to 400%).

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References

  1. Norwegian Scientific Committee for Food Safety (VKM) 2011, Evaluation of negative and positive health effects of n-3 fatty acids as constituents of food supplements and fortified foods. p 147

  2. Bimbo, A. West Virginia University Research Corporation Forum, 2013

  3. Rustad T, Storrø I, Slizyte R (2011) Possibilities for the utilisation of marine by-products. Int J Food Sci Technol 46:2001–2014

    Article  CAS  Google Scholar 

  4. Olafsen T, Richardsen R, Nystøyl R, Strandheim G, Kosmo JP (2012) Analyse av marint restråstoff, Fiskeri- og havbruksnæringens forskningsfond

  5. Bimbo, A. P. 2007 Processing of marine oils. Breivik, H., Ed. In Long-Chain Omega-3 speciality oils Vol. 21, The Oily Press: Bridgewater, EnglanD

  6. Xu X, H-Kittikun A, Zhang H (2007) Enzymatic processing of omega-3 speciality oils. In Long-chain omega-3 speciality oils, Breivik, H., Ed. The Oily Press: Bridgwater, England Vol. 21

  7. EFSA J (2010) European Food Safety Authority, Scientific Opinion on Fish Oil for Human Consumption. Food Hygiene, including Rancidity. EFSA Journal. 8, 1–48

  8. Laguerre M, Lecomte J, Villeneuve P (2007) Evaluation of the ability of antioxidants to counteract lipid oxidation: existing methods, new trends and challenges. Prog Lipid Res 46:244–282

    Article  CAS  Google Scholar 

  9. Frankel EN, Huang SW, Kanner J (1994) Bruce German, J., Interfacial phenomena in the evaluation of antioxidants: bulk oils vs emulsions. J Agric Food Chem 42:1054–1059

    Article  CAS  Google Scholar 

  10. Decker EA (1998) Antioxidant mechanisms. In: Akoh CC, Min DB (eds) Food lipids: chemistry, nutrition, and biotechnology. Marcel Dekker, New York, pp 397–421

    Google Scholar 

  11. Jacobsen C, Schwarz K, Stöckmann H, Meyer AS, Adler-Nissen J (1999) Partitioning of selected antioxidants in mayonnaise. J Agric Food Chem 47:3601–3610

    Article  CAS  Google Scholar 

  12. Schwarz K, Frankel EN, German JB (1996) Partition behaviour of antioxidative phenolic compounds in heterophasic systems. Lipid/Fett. 98:115–121

    Article  CAS  Google Scholar 

  13. Medina I, Alcántara D, González MaJ, Torres P, Lucas R, Roque J, Plou FJ, Morales JC (2010) Activity of resveratrol in several fish lipid matrices: effect of acylation and glucosylation. J Agric Food Chem 58:9778–9786

    Article  CAS  Google Scholar 

  14. Hamm W (2009) Processing of Fish Oil. In:fish oils, Rossell, B., Ed. Blackwell Publishing Ltd pp 81–98

  15. Carvajal, A. K.; Slizyte, R.; Storrø, I.; Aursand, M., Production of high quality fish oil by thermal treatment and enzymatic hydrolysis from fresh Norwegian spring spawning herring by-products. J Aquat Food Prod Technol (in press)

  16. Yarnpakdee S, Benjakul S, Nalinanon S, Kristinsson HG (2012) Lipid oxidation and fishy odour development in protein hydrolysate from Nile tilapia (Oreochromis niloticus) muscle as affected by freshness and antioxidants. Food Chem 132:1781–1788

    Article  CAS  Google Scholar 

  17. AOAC, Official Methods of Analysis. 1990

  18. Sosulski FW, Imafidon GI (1990) Amino acid composition and nitrogen-to-protein conversion factors for animal and plant foods. J Agric Food Chem 38:1351–1356

    Article  CAS  Google Scholar 

  19. Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 37:911–917

    Article  CAS  Google Scholar 

  20. Dauksas E, Falch E, Slizyte R, Rustad T (2005) Composition of fatty acids and lipid classes in bulk products generated during enzymic hydrolysis of cod (Gadus morhua) by-products. Process Biochem 40:2659–2670

    Article  CAS  Google Scholar 

  21. Mozuraityte R, Rustad T, Storrø I (2006) Pro-oxidant activity of Fe2+ in oxidation of cod phospholipids in liposomes. Eur J Lipid Sci Technol 108:218–226

    Article  CAS  Google Scholar 

  22. Carvajal AK, Rustad T, Mozuraityte R, Storrø I (2009) Kinetic studies of lipid oxidation induced by hemoglobin measured by consumption of dissolved oxygen in a liposome model system. J Agric Food Chem 57:7826–7833

    Article  CAS  Google Scholar 

  23. Mozuraityte R, Rustad T, Storrø I (2008) The role of iron in peroxidation of polyunsaturated fatty acids in liposomes. J Agric Food Chem 56:537–543

    Article  CAS  Google Scholar 

  24. Kristinová V, Mozuraityte R, Storrø I, Rustad T (2009) Antioxidant activity of phenolic acids in lipid oxidation catalyzed by different prooxidants. J Agric Food Chem 57:10377–10385

    Article  Google Scholar 

  25. AOAC 1994, AOAC Official Method 983.15.

  26. Method TTIPO2.01 AFD (2002) Peroxide Number of edible oils. In food and beverage analysis, Radiometer analytical SAS: Villerurbanne Cedex, France

  27. AOCS (1994), Official methods and recommended practices of the American Oil Chemists’ Society. 4 ed.; IL, AOCS: Champaign

  28. Nenadis N, Zafiropoulou I, Tsimidou M (2003) Commonly used food antioxidants: a comparative study in dispersed systems. Food Chem 82:403–407

    Article  CAS  Google Scholar 

  29. Frankel EN (2001) Interfacial Lipid Oxidation and Antioxidation. J Oleo Sci 50:387–391

    Article  CAS  Google Scholar 

  30. Porter WL, Black ED, Drolet AM (1989) Use of polyamide oxidative fluorescence test on lipid emulsions: contrast in relative effectiveness of antioxidants in bulk versus dispersed systems. J Agric Food Chem 37:615–624

    Article  CAS  Google Scholar 

  31. Kulås E, Olsen, E, Ackman R.(2003) Oxidation of fish lipids and its inhibition with tocopherols.Kamal-Eldin, A., Ed In Lipid oxidation pathways Vol.1 AOCS Press: Champaign, Ill., : pp VII, 323 s

  32. Waraho T, McClements DJ, Decker EA (2011) Mechanisms of lipid oxidation in food dispersions. Trends Food Sci Technol 22:3–13

    Article  CAS  Google Scholar 

  33. European Parliament and Council Directive No 95/2/EC of 20 February 1995 on food additives other than colours and sweeteners. In European Parliament and Council Directive, Ed Off J Eur Commun L61 p 1–40

  34. Kopelman IJ, Mizrahi S, Schab R (1975) Equilibrium vapor pressure of butylated hydroxyanisole and butylated hydroxytolune in high temperature oil solution. J Am Oil Chem Soc 52:103–105

    Article  CAS  Google Scholar 

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Acknowledgments

The authors wish to thank the Norwegian Research Council (project 173326), the Ministry of Foreign Affairs and EPAX (project “Sildeomega 3”) for financial support in carrying out the experiments and preparing this paper. Grøntvedt Pelagic is thanked for kindly providing herring by-product for the experiments and for making it possible to run the experiments near their filleting factory.

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Authors and Affiliations

  1. SINTEF Fisheries and Aquaculture, 7465, Trondheim, Norway

    Ana Karina Carvajal, Revilija Mozuraityte, Inger Beate Standal, Ivar Storrø & Marit Aursand

  2. Department of Biotechnology, Norwegian University of Science and Technology, Trondheim, Norway

    Ana Karina Carvajal & Marit Aursand

Authors
  1. Ana Karina Carvajal
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  2. Revilija Mozuraityte
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  3. Inger Beate Standal
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  4. Ivar Storrø
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  5. Marit Aursand
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Correspondence to Ana Karina Carvajal.

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Carvajal, A.K., Mozuraityte, R., Standal, I.B. et al. Antioxidants in Fish Oil Production for Improved Quality. J Am Oil Chem Soc 91, 1611–1621 (2014). https://doi.org/10.1007/s11746-014-2508-0

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  • DOI: https://doi.org/10.1007/s11746-014-2508-0

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