Abstract
The lipase from Burkholderia sp. EQ3 was used to synthesize wax esters in comparison with commercial lipases. The supernatant of Burkholderia sp. EQ3 was collected from a liquid basal medium with 1 % fish oil after 12 h cultivation (1.90 U/ml of lipase activity). The crude lipase was prepared by acetone precipitation of the culture supernatant (4.70 U/mg and 9.40 purification folds). The crude fish fat obtained by hexane extraction of waste fat from the wastewater pond of a canned tuna factory and cetyl alcohol were used for wax esters synthesis. Five commercial lipases were screened in comparison with crude lipase from Burkholderia sp. EQ3 in wax esters synthesis. The optimum conditions for wax esters synthesis from crude fish fat using Novozyme 435 were enzyme 1 U, substrate molar ratio of crude fish fat to cetyl alcohol 1:2 (115.30 mg of crude fish fat and 66.67 mg of cetyl alcohol) in hexane at 37 °C and 200 rpm with 90.81 % (TLC–FID peak area) after one h of reaction. The optimum conditions for the synthesis by crude lipase from Burkholderia sp. EQ3 were crude lipase 40 U, substrate molar ratio of crude fish fat and cetyl alcohol 1:2 in isooctane at 30 °C and 200 rpm with 95.07 % (TLC–FID peak area) after 6 h of reaction. The synthesized wax esters were mainly composed of cetyl palmitate and cetyl oleate.
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Acknowledgments
The research was supported by the Agro-Industry Practice School (APS) from the National Center for Genetic Engineering & Biotechnology (BIOTEC), National Science & Technology Development Agency. Financial support was also given by the Graduated School of Prince of Songkla University and the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission. The authors would like to thank Songkhla Canning Public Company Limited (Hat Yai, Thailand) for providing raw materials in this study.
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Ungcharoenwiwat, P., H-Kittikun, A. Synthesis of Wax Esters from Crude Fish Fat by Lipase of Burkholderia sp. EQ3 and Commercial Lipases. J Am Oil Chem Soc 90, 359–367 (2013). https://doi.org/10.1007/s11746-012-2183-y
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DOI: https://doi.org/10.1007/s11746-012-2183-y