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Enzymatic Synthesis of Diacylglycerols Enriched with Conjugated Linoleic Acid by a Novel Lipase from Malassezia globosa

  • Original Paper
  • Published:
Journal of the American Oil Chemists' Society

Abstract

Diacylglycerols (DAG) of conjugated linoleic acid (CLA) were prepared by esterification of glycerol with fatty acids enriched with CLA (FFA–CLA, >95%) in the presence of a novel lipase from Malassezia globosa (SMG1). Lipase SMG1 is strictly specific to mono- and diacylglycerols but not triacylglycerols, which is similar to the properties of lipase from Penicillium camembertii (lipase G 50), but lipase SMG1 showed preference on the production of DAG with the reaction proceeding. Low temperature was beneficial for the conversion of FFA–CLA into acylglycerols, the degree of esterification reached 93.0% when the temperature was 5 °C. The maximum DAG content (53.4%) was achieved at 25 °C. The rate of DAG synthesis increased as the enzyme loading increased. However, at lipase amounts above 240 U/g mixtures, no significant increases in DAG concentration were observed. The molar ratio of FFA–CLA to glycerol and initial water content were optimized to be 1:3 (mol/mol) and 3%. Lipase SMG1 showed no regioselectivity because the contents of 1,3-DAG and 1,2-DAG were 43.1% and 21.2% based on total content of acylglycerols. By calculating the ratio of 9c, 11t-CLA to 10t, 12c-CLA, it was indicated that lipase SMG1 showed a little preference to 10t, 12c-CLA at the sn-1(3) position of monoacylglycerols (MAG), while no selectivity for 9c, 11t-CLA at the sn-2 position of DAG was obviously found.

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Acknowledgments

This work was made possible with funding provided by the Guangdong Key Technology R&D Program (No. 2009B02020-1007), the Guangzhou Key Technology R&D Program (No. 2009A1-E021) and the Fundamental Research Funds for the Central Universities (2011ZZ0018).

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Authors and Affiliations

  1. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, People’s Republic of China

    Da Xu, Lijuan Sun, Huayong Chen & Bo Yang

  2. School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, 510640, People’s Republic of China

    Dongming Lan

  3. College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, 510640, People’s Republic of China

    Yonghua Wang

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  1. Da Xu
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  2. Lijuan Sun
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  3. Huayong Chen
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  4. Dongming Lan
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  5. Yonghua Wang
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Correspondence to Yonghua Wang or Bo Yang.

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Xu, D., Sun, L., Chen, H. et al. Enzymatic Synthesis of Diacylglycerols Enriched with Conjugated Linoleic Acid by a Novel Lipase from Malassezia globosa . J Am Oil Chem Soc 89, 1259–1266 (2012). https://doi.org/10.1007/s11746-012-2018-x

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  • DOI: https://doi.org/10.1007/s11746-012-2018-x

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