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Sulfatides Primarily Exist in the Substantia Nigra Region of Mouse Brain Tissue

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Lipids

Abstract

Lipid distribution in the brain is important for many biological functions and has been associated with some brain diseases. The aim of this study was to investigate lipid distribution in different regions of brain tissue in mice. To this end, substantia nigra (SN), caudate putamen (CPu), hippocampus (Hip), hypothalamus (Hyp), and cortex (Cx) tissues of mice were analyzed using direct infusion nanoelectrospray-ion trap mass spectrometry and multivariate analyses. The SN, CPu, Hip, Hyp, and Cx groups showed clear differences in lipid distribution using principal component analysis and a partial least-squares discriminant analysis score plot, and lipid levels were significantly different in different brain regions. In particular, sulfatides were mainly distributed in the SN region. Our results could be used to help understand the functions and mechanisms of lipids in various brain diseases.

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Abbreviations

SN:

Substantia nigra

CPu:

Caudate putamen

Hip:

Hippocampus

Hyp:

Hypothalamus

Cx:

Cortex

PCA:

Principal component analysis

PLS-DA:

Partial least-squares discriminant analysis

AD:

Alzheimer’s disease

TBI:

Traumatic brain injury

PL:

Phospholipid

SL:

Sphingolipid

PtdCho:

Phosphatidylcholine

PtdEtn:

Phosphatidylethanolamine

PakEtn:

Plasmanyl phosphatidylethanolamine

PlsEtn:

Plasmenyl phosphatidylethanolamine

PtdIns:

Phosphatidylinositol

PtdSer:

Phosphatidylserine

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Acknowledgements

This study was supported by the Bio-Synergy Research Project (Grants NRF-2014M3A9C4066459) and the Bio & Medical Technology Development Program (Grants NRF-2015M3A9E1028326 and NRF-2013M3A9B6046416) of the Ministry of Science, ICT & Future Planning through the National Research Foundation.

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Authors and Affiliations

  1. BK21 Plus KNU Multi-Omics Based Creative Drug Research Team, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu, 41566, Korea

    Kab-Tae Park, Jong Cheol Shon, Ji-Eun Kim & Kwang-Hyeon Liu

  2. College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu, 41566, Korea

    Gyu Hwan Park

  3. College of Pharmacy and Institute of Pharmaceutical Sciences, CHA University, Seongnam, 13488, Korea

    Hyun Jin Choi

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  1. Kab-Tae Park
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  2. Jong Cheol Shon
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  3. Ji-Eun Kim
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  4. Gyu Hwan Park
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Correspondence to Hyun Jin Choi or Kwang-Hyeon Liu.

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Park, KT., Shon, J.C., Kim, JE. et al. Sulfatides Primarily Exist in the Substantia Nigra Region of Mouse Brain Tissue. Lipids 52, 179–187 (2017). https://doi.org/10.1007/s11745-016-4224-z

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  • DOI: https://doi.org/10.1007/s11745-016-4224-z

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