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Olive Oil-Based Lipid Emulsions Do Not Influence Platelet Receptor Expression in Comparison to Medium and Long Chain Triglycerides In vitro

  • Original Article
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Lipids

Abstract

Lipid emulsions influence platelet aggregation and receptor expression. However, the effect on platelet function is not fully explained. Therefore, the aim of this study was to examine the influence of the lipids Lipofundin®, Lipidem® and ClinOleic® on surface expressions of P-selectin, GPIb and GPIIb/IIIa on platelets in vitro. Whole blood was incubated in two different concentrations (0.06 and 0.6 mg/ml) of LCT/MCT, n-3/LCT/MCT and LCT-MUFA for 30 min, followed by activation with TRAP-6 or ADP for flow-cytometric assay. Rates of P-selectin, GPIb and GPIIb/IIIa expression were analyzed. There was a significant increase in GPIIb/IIIa- and P-selectin-expression after incubation with LCT/MCT and n-3/LCT/MCT at the concentration of 0.6 mg/ml, without and after stimulation with TRAP-6 and ADP. GPIb was significantly decreased. Accordingly, LCT-MUFA had no effect on receptor expression of platelets in vitro. We demonstrated that LCT-MUFA did not activate receptor expression of platelets whereas LCT/MCT significantly increased platelet aggregation in vitro. This finding should be noted for parenteral nutrition of intensive care patients and, in the future, might provide further insight into the pathogenic pathways of acute thromboembolic events. However, prospectively designed clinical studies are needed to support our results.

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Abbreviations

ADP:

Adenosine-5′-diphosphate

BSA:

Bovine serum albumin

CLA:

Conjugated linoleic acid

FITC:

Fluorescein isothiocyanate

FO:

Fish oil

GPIb:

Glycoprotein Ib

GPIIb/IIIa:

Glycoprotein IIb/IIIa

IgG:

Immunoglobulin G

LCT:

Long-chain triglycerides

LCT/MCT:

Lipofundin®

LCT-MUFA:

Clinoleic®

MCT:

Medium-chain triglycerides

MUFA:

Monosaturated fatty acids

n-3/LCT/MCT:

Lipidem®

PBS:

Phosphate-buffered saline

PE:

Phycoerythrin

SFA:

Saturated fatty acid

SO:

Soybean oil

TPN:

Total parenteral nutrition

TRAP-6:

Thrombin Receptor activating peptide-6

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Authors and Affiliations

  1. Department of Anesthesiology and Intensive Care Medicine, Hannover Medical School, Carl-Neuberg Strasse 1, 30625, Hannover, Germany

    Carsten Stoetzer, Katja Nickel, Annette Weißig, Marieke Großheim, Dirk Scheinichen, Thorben Doll & Björn Jüttner

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  1. Carsten Stoetzer
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  2. Katja Nickel
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  3. Annette Weißig
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Correspondence to Carsten Stoetzer.

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Stoetzer, C., Nickel, K., Weißig, A. et al. Olive Oil-Based Lipid Emulsions Do Not Influence Platelet Receptor Expression in Comparison to Medium and Long Chain Triglycerides In vitro . Lipids 51, 1241–1248 (2016). https://doi.org/10.1007/s11745-016-4182-5

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  • DOI: https://doi.org/10.1007/s11745-016-4182-5

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