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Identification of Plasmalogen Cardiolipins from Pectinatus by Liquid Chromatography–High Resolution Electrospray Ionization Tandem Mass Spectrometry

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Lipids

Abstract

High resolution electrospray ionization tandem mass spectrometry (HR-ESI–MS/MS) was used to analyze cardiolipins (Ptd2Gro) including their plasmalogen forms from three species of the anaerobic beer-spoilage bacterial genus Pectinatus. Cardiolipins including their plasmalogens were analyzed by HR-ESI–MS/MS on Orbitrap and almost 100 cardiolipins (i.e. tetra-acyl—Ptd2Gro, plasmenyl-tri-acyl—PlsPtd2Gro, and di-plasmenyl-di-acyl—Pls2Ptd2Gro) of three classes were identified. The structures of the molecular species that consist of various regioisomers and structurally similar compounds were revealed in detail. The high resolution mass spectrometry allowed the unambiguous structural assignment of Ptd2Gro, PlsPtd2Gro, and Pls2Ptd2Gro in the three species of Pectinatus, which contain predominantly odd numbered fatty acids.

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Abbreviations

CID:

Collision-induced dissociation

DSMZ:

Deutsche Sammlung von Mikroorganismen und Zellkulturen

FA:

Fatty acid(s)

FAME:

Fatty acid methyl ester(s)

GC–MS:

Gas chromatography–mass spectrometry

HILIC:

Hydrophilic interaction liquid chromatography

HR-ESI–MS:

High resolution electro spray mass spectrometry

LC/ESI–MS/MS:

Liquid chromatography–electrospray ionization tandem mass spectrometry

PIsOH:

Phosphatidylinositol

Pls2Ptd2Gro:

Diplasmenyl-diacyl-cardiolipin

PlsPtd2Gro:

Plasmenyl-triacyl-cardiolipin

Ptd2Gro:

Cardiolipin

PtdEtn:

Phosphatidylethanolamine

PtdGro:

Phosphatidylglycerol

PtdOH:

Phosphatidic acid

RIBM:

Research Institute of Brewing and Malting

TLC:

Thin layer chromatography

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Acknowledgments

The research was supported by GACR P503/11/0215 and P503/12/1424, and by the Institutional Internal Project RVO 61388971.

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Authors and Affiliations

  1. Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 14220, Prague, Czech Republic

    Tomáš Řezanka & Karel Sigler

  2. Research Institute of Brewing and Malting, Lípová 511/15, 12000, Prague, Czech Republic

    Dagmar Matoulková

  3. Department of Biotechnology, Institute of Chemical Technology Prague, Technická 5, 16628, Prague, Czech Republic

    Lucie Kyselová

Authors
  1. Tomáš Řezanka
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  2. Dagmar Matoulková
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  3. Lucie Kyselová
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  4. Karel Sigler
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Correspondence to Tomáš Řezanka.

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Řezanka, T., Matoulková, D., Kyselová, L. et al. Identification of Plasmalogen Cardiolipins from Pectinatus by Liquid Chromatography–High Resolution Electrospray Ionization Tandem Mass Spectrometry. Lipids 48, 1237–1251 (2013). https://doi.org/10.1007/s11745-013-3851-x

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