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Diet and lipoprotein oxidation: Analysis of oxidized triacylglycerols in pig lipoproteins

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Lipids

Abstract

Oxidized lipoproteins have a recognized role in atherogenesis, but molecular-level research on oxidized lipids in lipoproteins and the effect of diet on these molecules have been limited. In the present study, the effects of three sunflower seed oil diets differing in oxidation levels (PV in oils 1, 84, and 223 mequiv O2/kg) on lipoprotein lipid oxidation in growing pigs were investigated. The emphasis was on the investigation of oxidized TAG molecules found in chylomicrons and VLDL. A method based on RP-HPLC and electrospray ionization-MS was used for the analysis of oxidized TAG molecules. The baseline diene conjugation method was used for the estimation of in vivo levels of lipoprotein lipid oxidation. Several oxidized TAG structures were found in the samples. These products consisted of TAG molecules with a hydroxy, an epoxy, or a keto group attached to a FA, and of TAG molecules containing an aldehyde structure derived from a FA. The lipoprotein lipids and TAG were more oxidized in the pigs fed on the most oxidized oil compared with those fed on nonoxidized oil. Oxidation of dietary fat was reflected in the lipoprotein oxidation. New, detailed information on oxidized TAG molecules of chylomicrons and VLDL was obtained.

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Abbreviations

ACN:DB:

acyl carbon number:number of double bonds

BDC:

baseline diene conjugation

DNPH:

2,4-dinitrophenyl hydrazine

ESI:

electrospray ionization

TPP:

triphenyl phosphine

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Authors and Affiliations

  1. Department of Biochemistry and Food Chemistry, University of Turku, FIN-20014, Turku, Finland

    Jukka-Pekka Suomela, Olli Sjövall, Juha-Pekka Kurvinen & Heikki Kallio

  2. MCA Research Laboratory, Department of Physiology, University of Turku, FIN-20014, Turku, Finland

    Markku Ahotupa

  3. Department of Biochemistry and Pharmacy, Åbo Akademi University, FIN-20521, Turku, Finland

    Olli Sjövall

  4. Functional Foods Forum, University of Turku, PIN-20520, Turku, Finland

    Heikki Kallio

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  1. Jukka-Pekka Suomela
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  2. Markku Ahotupa
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  4. Juha-Pekka Kurvinen
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  5. Heikki Kallio
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Correspondence to Jukka-Pekka Suomela.

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Suomela, JP., Ahotupa, M., Sjövall, O. et al. Diet and lipoprotein oxidation: Analysis of oxidized triacylglycerols in pig lipoproteins. Lipids 39, 639–647 (2004). https://doi.org/10.1007/s11745-004-1277-4

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  • DOI: https://doi.org/10.1007/s11745-004-1277-4

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