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Cadmium effect on hydrogen peroxide, gluthatione and phytochelatins levels in potato tuber

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Abstract

Short-term treatment of potato tuber (Solanum tuberosum L.) dises with CdCl2 (1mM) induced an oxidative stress, manifested by higher levels of H2O2, and activated the synthesis of phytochclatins ((γ-Glu-Cys)n-Gly): PC2, PC3 and PC4. If in the tissues with a lower GSH level, the oxidative stress was induced by treatment with 3-aminotriazol (AT), or with AT and H2O2, the elevation of H2O2 and GSH levels and then some accumulation of thiols, including PC2, PC3 and PC4, were observed. However, this increase of PC concentration was considerably lower when compared with the effects brought about by Cd+2 treatment. If such a procedure of evoking subsequent moderate oxidative stress in tissues preceded Cd-treatment, a marked limitation of PC synthesis was observed. The presented results support the role of H2O2 as the second messenger in activating GSH synthesis and thus suggest a possibility of redox type regulation mechanism of PCs synthesis.

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Abbreviations

AOS:

active oxygen species

AT:

3-amino-1,2,4-triazol

BSO:

buthionine sulfoximine

CAT:

catalase

CdS :

soluble Cd content

CdB :

bound cadmium content

GSH:

glutathione

HPLC:

high performance liquid chromatography

MD:

menadione

OS:

oxidative stress

PCn :

phytochelatins

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Authors and Affiliations

  1. Department of Plant Physiology, Agricultural University, Wolyńska 35, 60637, Poznań, Poland

    Andrzej Stroiński & Malgorzata Zielezińska

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  1. Andrzej Stroiński
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  2. Malgorzata Zielezińska
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Stroiński, A., Zielezińska, M. Cadmium effect on hydrogen peroxide, gluthatione and phytochelatins levels in potato tuber. Acta Physiol Plant 19, 127–135 (1997). https://doi.org/10.1007/s11738-997-0029-2

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  • DOI: https://doi.org/10.1007/s11738-997-0029-2

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