Abstract
Water deficit is one of the major factors affecting the growth and productivity field crops. This study was conducted to characterize four popularly genotypes, viz. SGC 16, TMB 37, SG 21-5, and Pratap, of green gram (Vigna radiata L.) on physiological, biochemical and morphological basis under water deficit at vegetative, flowering and pod filling stages. A substantial decrease in the mid-day leaf water potential (ΨL), net photosynthesis (P N), total soluble protein (TSP) and membrane stability index (MSI) were recorded under water deficit. However, the membrane lipid peroxidation (MDA), total free amino acid, and the activities of super oxide dismutase and catalase increased significantly (p ≤ 0.05). Percentage reduction of grain yield, under water deficit, was the maximum in the genotype TMB 37 (50.05–63.80 %) indicating its sensitivity towards water deficit. In contrast, the genotype Pratap was the most tolerant to water deficit as it had the minimum reduction (20.76–34.87 %) in grain yield. Flowering was the most critical crop growth stage to water deficit. Among the studied parameters, ΨL, P N, TSP, MSI and MDA were identified as the marker parameters for explaining the response mechanism of green gram genotypes to water deficit.
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References
Abedi T, Pakniyat H (2010) Antioxidant enzyme changes in response to drought stress in ten cultivars of oilseed rape (Brassica napus L.). Czech J Genet Plant Breed 46:27–34
Aebi H (1984) Catalase in vitro. Method Enzym 105:121–126
Ahmad ST, Haddad R (2011) Study of silicon effects on antioxidant enzyme activities and osmotic adjustment of wheat under drought stress. Czech J Genet Plant Breed 47:17–27
Ali M, Gupta S (2012) Carrying capacity of Indian agriculture: pulse crops. Curr Sci 102:874–881
Bano Q, Ilyas N, Bano A, Zafar N, Akram A, Hassan F (2013) Effect of azospirillum inoculation on maize (zea mays L.) under drought stress. Pak J Bot 45:13–20
Baroowa B, Gogoi N (2016a) The effect of osmotic stress on anti-oxidative capacity of black gram (Vigna mungo L.). Exp Agric. doi:10.1017/S0014479716000090
Baroowa B, Gogoi N (2016b) Morpho-physiological and yield responses of black gram (Vigna mungo L.) and green gram (Vigna radiata L.) genotypes under drought at different growth stages. Res J Recent Sci 5:43–50
Baroowa B, Gogoi N, Paul S, Baruah KK (2015) Response of leaf water status, stomatal characteristics, photosynthesis and yield in black gram and green gram genotypes to soil water deficit. Funct Plant Biol 42:1010–1018
Bartlett MK, Scoffoni C, Sack L (2012) The determinants of leaf turgor loss point and prediction of drought tolerance of species and biomes: a global meta-analysis. Ecol Lett 15:393–405
Baskaran L, Sundaramoorthy P, Chidambaram ALA, Sankar Ganesh K (2009) Growth and physiological activity of green gram (Vigna radiata L.) under effluent stress. Bot Res Intl 2:107–114
Batra NG, Sharma V, Kumari N (2014) Drought-induced changes in chlorophyll fluorescence, photosynthetic pigments, and thylakoid membrane proteins of Vigna radiata. J Plant Interact 9:712–721
Chernane H, Latique S, Mansori M, El Kaoua M (2015) Salt stress tolerance and antioxidative mechanisms in wheat plants (Triticum durum L.) by seaweed extracts application. J Agricul Vet Sci 8:36–44
Corpas FJ, Palma JM, Sandalio LM, Valderrama R, Barroso JB, del Río LA (2008) Peroxisomal xanthine oxidoreductase: characterization of the enzyme from pea (Pisum sativum L.) leaves. J Plant Physiol 165:1319–1330
De Diego N, Pérez-Alfocea F, Cantero E, Lacuesta M, Moncaleán P (2012) Physiological response to drought in radiata pine: phytohormone implication at leaf level. Tree Physiol 32:435–449
De Diego N, Sampedro MC, Barrio RJ, Saiz-Fernández I, Moncaleán P, Lacuesta M (2013) Solute accumulation and elastic modulus changes in six radiata pine breeds exposed to drought. Tree Physiol 33:69–80
de Souza TC, de Castro EM, Magalhães PC, Lino LD, Alves ET, de Albuquerque PE (2013) Morphophysiology, morphoanatomy, and grain yield under field conditions for two maize hybrids with contrasting response to drought stress. Acta Physiol Plant 35:3201–3211
Dhindsa RH, Dhindsa P, Thorpe TA (1981) Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased level of SOD and CAT. J Exp Bot 32:93–101
Din J, Khan SU, Ali I, Gurmani AR (2011) Physiological and agronomic response of canola varieties to drought stress. J Anim Plant Sci 21:78–82
Farooq M, Wahid A, Kobayashi N, Fujita D, Basra SMA (2009) Plant drought stress: effects, mechanisms and management. Agron Sustain Dev 29:185–212
Farooq M, Gogoi N, Barthakur S, Baroowa B, Bharadwaj N, Alghamdi SS, Siddique KHM (2016) Drought stress in grain legumes during reproduction and grain filling. J Agron Crop Sci. doi:10.1111/jac.12169
Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48:909–930
Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198
Hu J, Jiang D, Cao W, Luo W (2004) Effect of short-term drought on leaf water potential, photosynthesis and dry matter partitioning in paddy rice. J Appl Ecol 15:63–67
Huseynova IM (2012) Photosynthetic characteristics and enzymatic antioxidant capacity of leaves from wheat cultivars exposed to drought. Biochim Biophys Acta 1817:1516–1523
Khan N, Naqvi FN (2010) Effect of water stress on lipid peroxidation and antioxidant enzymes in local bread wheat hexaploids. J Food Agric Environ 8:521–526
Liang W, Wang L, Shi J, Lei X, Yang J, Wu S, Chen W (2014) Differential expression of antioxidant proteins in the drought-tolerant cyanobacterium Nostoc flagelliforme under desiccation. Plant Omics 7:205–212
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Mafakheri A, Siosemardeh A, Bahramnejad B, Struik PC, Sohrabi Y (2011) Effect of drought stress and subsequent recovery on protein, carbohydrate contents, catalase and peroxidase activities in three chickpea (Cicer arietinum) cultivars. Aust J Crop Sci 5:1255–1260
Masoumi H, Darvish F, Daneshian J, Ghorban N, Habibi D (2011) Effects of water deficit stress on seed yield and Antioxidants content in soybean (Glycine max L.) cultivars. Afr J Agric Res 6:1209–1218
Meng X, Yin B, Feng HL, Zhang S, Liang XQ, Meng QW (2014) Overexpression of R2R3-MYB gene leads to accumulation of anthocyanin and enhanced resistance to chilling and oxidative stress. Biol Plant 58:121–130
Moradi Dezfuli P, Sharif-zadeh F, Janmohammadi M (2008) Influence of priming techniques on seed germination behavior of maize inbred lines (Zea mays L.). Arpn J Agric Biol Sci 3(3):22–25
Osakabe Y, Yamaguchi-Shinozaki K, Shinozaki K, Tran LS (2013) Sensing the environment: key roles of membrane-localized kinases in plant perception and response to abiotic stress. J Exp Bot 64:445–458
Pandey HC, Baig MG, Chandra A, Bhatt RK (2010) Drought stress induced changes in lipid peroxidation and antioxidant system in genus Avena. J Environ Biol 31:435–440
Premachandra GS, Saneoka H, Ogata S (1990) Cell membrane stability, an indicator of drought tolerance, as affected by applied nitrogen in soybean. J Agr Sci 115:63–66
Sairam RK (1994) Effect of moisture stress on physiological activities of two contrasting wheat genotypes. Ind J Exp Biol 32:594–597
Sairam RK, Srivastava GC, Saxena DC (2000) Increased antioxidant activity under elevated temperatures: a mechanism of heat stress tolerance in wheat genotypes. Biol Plant 43:245–251
Sandhya V, SkZ Ali, Grover M, Reddy G, Venkateswarlu B (2010) Effect of plant growth promoting Pseudomonas spp. on compatible solutes, antioxidant status and plant growth of maize under drought stress. Plant Growth Regul 62:21–30
Sayfzadeh S, Rashidi M (2010) Effect of drought stress on antioxidant enzyme activities and root yield of sugar beet (Beta vulgaris). Ame-Eurasian J Agri Environ Sci 9:223–230
Singh RK, Singh AN, Ram H, Prasad SR (2014) Growth, yield attributes and quality of summer green gram (Vigna radiata L.) as influenced by nitrogen and irrigation levels. Ann Agric Res 35:47–53
Surendar KK, Devi DD, Ravi I, Jeyakumar P, Velayudham K (2013) Water stress affects plant relative water content, soluble protein, total chlorophyll content and yield of ratoon banana. Int J Hortic 3:96–103
Thangavel P, Anandan A, Eswaran R (2011) AMMI analysis to comprehend genotype-by-environment (GE) interactions in rainfed grown mung bean (‘Vigna radiata’ L.). Aust J Crop Sci 5:1767–1775
Zlatev Z, Lidon FC (2012) An overview on drought induced changes in plant growth, water relations and photosynthesis. Emir J Food Agric 24:57–72
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Baroowa, B., Gogoi, N. & Farooq, M. Changes in physiological, biochemical and antioxidant enzyme activities of green gram (Vigna radiata L.) genotypes under drought. Acta Physiol Plant 38, 219 (2016). https://doi.org/10.1007/s11738-016-2230-7
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DOI: https://doi.org/10.1007/s11738-016-2230-7