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Isolation and in silico characterization of a shikimate kinase from Cassia obtusifolia

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Abstract

Shikimate kinase (SK), an indispensable enzyme in shikimate pathway, catalyzes the transfer of a phosphate from Adenosine triphosphate (ATP) to 3-hydroxyl of shikimate to form shikimate 3-phosphate. There are many active metabolites from shikimate pathway in Cassia obtusifolia. A new member of SKs from C. obtusifolia named CoSK was cloned and subjected to in silico characterization analysis. The constructed 3D structure of CoSK adopted α-β-α fold with five parallel β-sheets flanked by 12 α-helices. CoSK was shown to possess the potential ability to catalyze the phosphorylation of shikimate. Residues Lys118 and Arg223 binding with ATP and residue Asp137 binding with shikimate might be essential for phosphorylating shikimate. These results will provide useful information concerning the catalytic and physiology mechanism of SK in plants.

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Abbreviations

SKM:

Shikimate

SK:

Shikimate kinase

ATP:

Adenosine triphosphate

NB:

Nucleotide binding

ESB:

Extended shikimate binding

DAF:

Days after flower

EM:

Energy minimization

MD:

Molecular dynamics

RMSD:

Root mean square deviation

RT-PCR:

Reverse transcriptase-polymerase chain reaction

cTP:

Chloroplast transit peptide

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Acknowledgments

This work was supported by the grant from National Natural Science Foundation of China (No. 31371232) and National Science and Technology Major Project of China (No. 2014ZX09304307001-019).

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    Authors and Affiliations

    1. School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, China

      Zubi Liu, Yangyang Li, Jihua Yu, Wanjun Wang, Rui Tan, Jiayu Zhou & Hai Liao

    2. College of Life Sciences, Zhejiang University, Hangzhou, 310058, China

      Qiankun Zhu

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    1. Zubi Liu
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    Correspondence to Jiayu Zhou or Hai Liao.

    Additional information

    Communicated by L.A. Kleczkowski.

    Z. Liu, Q. Zhu contributed equally.

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    Liu, Z., Zhu, Q., Li, Y. et al. Isolation and in silico characterization of a shikimate kinase from Cassia obtusifolia . Acta Physiol Plant 37, 85 (2015). https://doi.org/10.1007/s11738-015-1822-y

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    • DOI: https://doi.org/10.1007/s11738-015-1822-y

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