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Identification of changes in Triticum aestivum L. leaf proteome in response to drought stress by 2D-PAGE and MALDI-TOF/TOF mass spectrometry

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Abstract

Drought is an abiotic stress that strongly influences plant growth, development and productivity. To gain a better understanding of the drought-stress responses at physiological and molecular level in wheat plants (Triticum aestivum cv. KTC86211), we performed a comparative physiological and proteomics analysis. Eight-day-old wheat seedlings were treated with polyethylene glycol-simulated drought stress for 0, 24, 48 and 72 h. Drought treatment resulted in alterations of morphology, increased relative electrolyte leakage and reduced length and weight on leaf and root. Stress-induced proteome changes were analyzed by two-dimensional gel electrophoresis in conjunction with MALDI-TOF/TOF. Twenty-three spots differed significantly between control and treated plants following 48 h of drought stress, with 19 upregulated, and 4 downregulated, in leaf tissues. All of the differentially expressed protein spots were identified, revealing that the majority of proteins altered by drought treatment were involved in reactive oxygen species scavenging enzymes and photosynthesis. Other proteins identified were involved in protein metabolism, cytoskeleton structure, defense response, acid metabolism and signal transduction. All proteins might contribute cooperatively to reestablish cellular homeostasis under drought stress. The present study not only provides new insights into the mechanisms of acclimation and tolerance to drought stress in wheat plants, but also provides clues for improving wheat’s drought tolerance through breeding or genetic engineering.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31071349), the Research Fund for the Doctoral Program of Higher Education of China (20120204110033) and National Basic Scientific Research Foundation of China (Grant No. QN2013033). Seeds of wheat were generously provided by the Department of Botany, National University of Washington, USA.

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  1. State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi, People’s Republic of China

    Hongmei Zhang, Linsheng Zhang, Hui Lv, Zhengyang Yu, Dapeng Zhang & Weining Zhu

  2. College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, Henan, People’s Republic of China

    Hongmei Zhang

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  1. Hongmei Zhang
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  2. Linsheng Zhang
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Correspondence to Linsheng Zhang.

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Communicated by M. Hajduch.

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Zhang, H., Zhang, L., Lv, H. et al. Identification of changes in Triticum aestivum L. leaf proteome in response to drought stress by 2D-PAGE and MALDI-TOF/TOF mass spectrometry. Acta Physiol Plant 36, 1385–1398 (2014). https://doi.org/10.1007/s11738-014-1517-9

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