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Impact of low and high fluence rates of UV-B radiation on growth and oxidative stress in Phormidium foveolarum and Nostoc muscorum under copper toxicity: differential display of antioxidants system

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Abstract

In the present study, impact of low (UV-BL) and high (UV-BH) fluence rates of UV-B on growth, oxidative stress and antioxidant system was studied in two cyanobacteria i.e. Phormidium foveolarum and Nostoc muscorum under Cu (2 and 5 μM) toxicity after 24 and 72 h of experiments. UV-BH and Cu treatment decreased growth of both the cyanobacteria and Cu induced decrease in growth was accompanied by a significant increase in Cu accumulation. Levels of reactive oxygen species (ROS), i.e. superoxide radicals (SOR; \( \text O_{2}^{\cdot\,-} \)) and hydrogen peroxide (H2O2) were significantly increased by Cu and UV-BH exposure which in turn accelerated lipid peroxidation (malondialdehyde: MDA) and protein oxidation (reactive carbonyl groups: RCG). Activities of enzymatic antioxidants, such as superoxide dismutase (SOD), peroxidase (POD) and glutathione-S-transferase (GST) were increased by both doses of Cu as well as UV-B. Conversely, Cu and UV-BH drastically decreased catalase (CAT) activity. After the commencement of 24 h of treatment with Cu alone and together with UV-BH, non-protein thiols (NP-SH) contents were decreased while after 72 h, a reverse trend was noticed. Unlike NP-SH, cysteine content decreased appreciably during the treatments. In contrast to this, low dose (UV-BL) of UV-B did not influence growth, SOR, H2O2, MDA and RCG contents. An improvement in CAT activity and NP-SH content was observed under Cu and UV-BL treatment; hence, UV-BL treatment resulted into certain degree of protection against Cu toxicity in both the organisms. Thus, the results showed that UV-BH and UV-BL exerted differential effects on both the organisms under Cu toxicity, and compared to N. muscorum, P. foveolarum was less affected by Cu and UV-BH.

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Acknowledgments

The authors wish to extend their thanks to The Head, Department of Botany, University of Allahabad, Allahabad for providing necessary lab facilities. The authors are also thankful to UGC, New Delhi, for financial support.

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  1. Ranjan Plant Physiology and Biochemistry Laboratory, Department of Botany, University of Allahabad, Allahabad, 211002, India

    Vijay Pratap Singh, Prabhat Kumar Srivastava & Sheo Mohan Prasad

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  1. Vijay Pratap Singh
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  2. Prabhat Kumar Srivastava
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Correspondence to Sheo Mohan Prasad.

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Communicated by K. Trebacz.

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Singh, V.P., Srivastava, P.K. & Prasad, S.M. Impact of low and high fluence rates of UV-B radiation on growth and oxidative stress in Phormidium foveolarum and Nostoc muscorum under copper toxicity: differential display of antioxidants system. Acta Physiol Plant 34, 2225–2239 (2012). https://doi.org/10.1007/s11738-012-1023-x

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  • DOI: https://doi.org/10.1007/s11738-012-1023-x

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