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Oxalic acid and oxalate oxidase enzyme in Costus pictus D. Don

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Abstract

The leaves of Costus pictus are sour in taste due to the presence of oxalic acid in the leaves. Different stages of leaves were collected and the samples were designated as stage one, stage two and stage three. It was found that oxalate content and oxalate oxidase activity were maximum in second leaf stage followed by first leaf stage and third leaf stage. Drying causes substantial loss of oxalate content and complete loss of oxalate oxidase activity. With various solvents water recovered more oxalate followed by methanol and ethanol while oxalate oxidase activity was maximum in ethanol followed by methanol and water. The ethanol or methanol extract of second leaf stage of C. pictus can be used for isolating active principles. The oxalate oxidase from C. pictus can be used as a cheap source of oxalate oxidase enzyme which is used in oxalate determination in biological fluids. Moreover, the sensitivity of oxalate determination employing oxalate oxidase from C. pictus will be more as oxalate oxidase in C. pictus has K m 20 times lesser than the oxalate oxidase enzyme from barley seedling.

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Acknowledgments

We thank Dr. Bhabesh Dutta, Post Doctoral Associate, Department of Plant Pathology, Coastal Plain Research Station, University of Georgia, Tifton, for providing the valuable references for this study. We also thank Dr. C. Vijayalakshmi, Professor and Head, Department of Crop Physiology, Tamil Nadu Agricultural University, Coimbatore, for giving valuable suggestions in manuscript preparation.

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Authors and Affiliations

  1. Department of Crop Physiology, Tamil Nadu Agricultural University, Coimbatore, 641 003, Tamil Nadu, India

    Rajendran Sathishraj

  2. Centre for Plant Biotechnology and Molecular Biology, College of Horticulture, Vellanikkara, Thrissur, 680 656, Kerala, India

    Antoney Augustin

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  1. Rajendran Sathishraj
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  2. Antoney Augustin
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Correspondence to Rajendran Sathishraj.

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Communicated by L. A. Kleczkowski.

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Sathishraj, R., Augustin, A. Oxalic acid and oxalate oxidase enzyme in Costus pictus D. Don. Acta Physiol Plant 34, 657–667 (2012). https://doi.org/10.1007/s11738-011-0866-x

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  • DOI: https://doi.org/10.1007/s11738-011-0866-x

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