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Raffinose in seedlings of winter vetch (Vicia villosa Roth.) under osmotic stress and followed by recovery

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Abstract

During germination of winter vetch (Vicia villosa Roth.) seeds, the degradation of raffinose family oligosaccharides and galactosyl pinitols occurred faster in axis than in cotyledons. After 7 days of germination, all α-d-galactosides disappeared and the soluble carbohydrates in seedling tissues consisted of d-pinitol, sucrose, fructose, glucose and myo-inositol. Osmotic stress caused by incubation of seedlings in PEG 8000 solution (−0.5, −1.0, and −1.5 MPa) for 48 h induced the activity of crucial enzymes of the RFOs pathway, i.e. galactinol synthase and raffinose synthase, in both the root and epicotyl but not in cotyledons. The root and epicotyl accumulated elevated amounts of galactinol and raffinose as the osmotic potential was lowered. This process was transient because when PEG solution was replaced with water, galactinol and raffinose were degraded, thus confirming their direct involvement in the response of tissues to osmotic stress. Among other soluble carbohydrates, only sucrose accumulated in response to stress. The results did not show potential role of d-pinitol in the adjustment of winter vetch seedlings to osmotic stress.

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Acknowledgments

This work was partially supported by grant No. N30312532/4015 obtained from the Ministry of Scientific Research and Information Technology in Poland. The authors wish to thank Dr Joanna Goszczyńska for technical assistance in experiments.

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Authors and Affiliations

  1. Department of Plant Physiology and Biotechnology, University of Warmia and Mazury in Olsztyn, ul. Oczapowskiego 1A/103A, 10-718, Olsztyn, Poland

    Lesław B. Lahuta & Ryszard J. Górecki

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  1. Lesław B. Lahuta
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  2. Ryszard J. Górecki
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Correspondence to Lesław B. Lahuta.

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Communicated by R. Aroca.

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Lahuta, L.B., Górecki, R.J. Raffinose in seedlings of winter vetch (Vicia villosa Roth.) under osmotic stress and followed by recovery. Acta Physiol Plant 33, 725–733 (2011). https://doi.org/10.1007/s11738-010-0597-4

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  • DOI: https://doi.org/10.1007/s11738-010-0597-4

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