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Functional characterization of a thermostable methionine adenosyltransferase from Thermus thermophilus HB27

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Abstract

MATTt (a thermostable methionine adenosyltransferase from Thermus thermophilus HB27) was overexpressed in Escherchia coli and purified using Ni-NTA affinity column. The enzymatic activity of MATTt was investigated in a temperature range from 30 °C to 90 °C, showing that MATTt exhibited a high enzymatic activity and good thermostability at 80 °C. Circular dichroism spectra reveals that MATTt contains high portion of β-sheet structures contributing to the thermostability of MATTt. The kinetic parameter, K m is 4.19 mmol/L and 1.2 mmol/L for ATP and methionine, respectively. MATTt exhibits the highest enzymatic activity at pH 8. Cobalt (Co2+) and zinc ion (Zn2+) enhances remarkably the activity of MATTt compared to the magnesium ion (Mg2+). All these results indicated that the thermostable MATTt has great potential for industry applications.

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References

  1. Catoni G L. S-Adenosylmethionine: A new intermediate formed enzymatically from L-methionine and adenosinetriphosphate. Journal of Biological Chemistry, 1953, 204: 403–416

    CAS  Google Scholar 

  2. Fontecave M, Atta M, Mulliez E. S-adenosylmethionine: Nothing goes to waste. Trends in Biochemical Sciences, 2004, 29(5): 243–249

    Article  CAS  Google Scholar 

  3. Momparler R L, Bovenzi V. DNA methylation and cancer. Journal of Cellular Physiology, 2000, 183: 145–154

    Article  CAS  Google Scholar 

  4. Dording C M, Mischoulon D, Shyu I. SAMe and sexual functioning. European Psychiatry, 2012, 27(6): 451–454

    Article  CAS  Google Scholar 

  5. Friedel H A, Goa K L, Benfield P. S-adenosyl-L-methionine: A review of its pharmacological properties and therapeutic potential in liver dysfunction and affective disorders in relation to its physiological role in cell metabolism. Drugs, 1989, 38: 389–416

    Article  CAS  Google Scholar 

  6. Lieber C S. S-adenosyl-L-methionine: Its role in the treatment of liver disorders. American Journal of Clinical Nutrition, 2002, 76: 1183S–1187S

    CAS  Google Scholar 

  7. Lu Z J, Markham G D. Enzymatic properties of S-adenosylmethionine synthetase from the archaeon Methanococcus jannaschii. Journal of Biological Chemistry, 2002, 277: 16624–16631

    Article  CAS  Google Scholar 

  8. Komoto J, Yamada T, Takata Y. Crystal structure of the Sadenosylmethionine synthetase ternary complex: A novel catalytic mechanism of S-adenosylmethionine synthesis from ATP and Met. Biochemistry, 2004, 4: 1821–1831

    Article  Google Scholar 

  9. Porcelli M, Caccilapuoti G, Carteni-farina M, Gambacorta A. Sdenosylmethionine synthetase in the thermophilic archaebacterium Sulfolobus solfataricus. Purification and characterization of two isoforms. European Journal of Biochemistry, 1988, 177: 273–280

    Article  CAS  Google Scholar 

  10. Garrido F, Estrela S, Alves S. Refolding and characterization of methionine adenosyltransferase from Euglena gracilis. Protein Expression and Purification, 2011, 79: 128–136

    Article  CAS  Google Scholar 

  11. Markham G D, Deparsis J, Gatmaitan J. The sequence of metK, the structural gene for S-adenosylmethionine synthetase in Escherichia coli. Journal of Biological Chemistry, 1984, 259: 14505–14507

    CAS  Google Scholar 

  12. Yoon G S, Ko K H, Kang H W. Characterization of Sadenosylmethionine synthetase from Streptomyces avermitilis NRRL8165 and its effect on antibiotic production. Enzyme and Microbial Technology, 2006, 39(3): 466–473

    Article  CAS  Google Scholar 

  13. Slapeta J, Stejskal F, Keithly J S. Characterization of sadenosylmethionine synthetase in Cryptosporidium parvum (apicomplexa). FEMS Microbiology Letters, 2003, 225(2): 271–277

    Article  CAS  Google Scholar 

  14. Kamarthapu V, Rao K V, Srinivas P N B S, Reddy G B, Reddy V D. Structural and kinetic properties of Bacillus subtilis s-adenosylmethionine synthetase expressed in Escherichia coli. Biochimica et Biophysica Acta, 2008, 1784(12): 1949–1958

    Article  CAS  Google Scholar 

  15. Burk M J, Van Dien S. Biotechnology for chemical production: Challenges and opportunities. Trends in Biotechnology, 2015, 34 (3): 187–190

    Article  Google Scholar 

  16. Oshima T, Imahori K. Description of Thermus thermophilus (Yoshida and Oshima) comb. nov., a nonsporulating thermophilic bacterium from a Japanese thermal spa. International Journal of Systematic Bacteriology, 1974, 24: 102–112

    Article  CAS  Google Scholar 

  17. Liu Y H, Song J N, Tan T W, Liu L. Production of fumaric acid from l-malic acid by solvent engineering using a recombinant thermostable fumarase from Thermus thermophilus HB8. Applied Biochemistry and Biotechnology, 2015, 175(6): 2823–2831

    Article  CAS  Google Scholar 

  18. Henne A, Brüggemann H, Raasch C. The genome sequence of the extreme thermophile Thermus thermophilus. Nature Biotechnology, 2004, 22: 547–553

    Article  CAS  Google Scholar 

  19. Chang Y L, Hsieh C L, Huang Y M. Modified method for determination of sulfur metabolites in plant tissues by stable isotope dilution-based liquid chromatography-electrospray ionization-tandem mass spectrometry. Analytical Biochemistry, 2013, 442: 24–33

    Article  CAS  Google Scholar 

  20. Carolina P, Miguel A A. K2D2: Estimation of protein secondary structure from circular dichroism spectra. BMC Structural Biology, 2008, 8: 25

    Article  Google Scholar 

  21. Whitmore L, Wallace B A. Protein secondary structure analyses from circular dichroism spectroscopy: Methods and reference databases. Biopolymers, 2008, 89: 392–400

    Article  CAS  Google Scholar 

  22. Thompson J D, Gibson T J, Plewniak F. The CLUSTAL_X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 1997, 25: 4876–4882

    Article  CAS  Google Scholar 

  23. Page R D. TreeView: An application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences: CABIOS, 1996, 12(4): 357–358

    CAS  Google Scholar 

  24. Deckert G, Warren P V, Gaasterland T. The complete genome of the hyperthermophilic bacterium Aquifex aeolicus. Nature, 1998, 392: 353–358

    Article  CAS  Google Scholar 

  25. Graham D E, Bock C L, Schalk-Hihi C, Lu Z J, Markham G D. Identification of a highly diverged class of S-adenosylmethionine synthetases in the archaea. Journal of Biological Chemistry, 2000, 275: 4055–4059

    Article  CAS  Google Scholar 

  26. Markham G D, Hafner E W, Tabor C W. S-Adenosylmethionine synthetase from Escherichia coli. Journal of Biological Chemistry, 1980, 255: 9082–9092

    CAS  Google Scholar 

  27. Kotb M, Kredich N M. S-Adenosylmethionine synthetase from human lymphocytes. Purification and characterization. Journal of Biological Chemistry, 1985, 260: 3923–3930

    CAS  Google Scholar 

  28. Takusagawa F, Kamitori S, Misaki S. Crystal structure of Sadenosylmethionine synthetase. Journal of Biological Chemistry, 1996, 271: 136–147

    Article  CAS  Google Scholar 

  29. Luo Y, Yuan Z, Luo G. Expression of secreted His-tagged Sadenosylmethionine synthetase in the methylotrophic yeast Pichia pastoris and its characterization, one-step purification, and immobilization. Biotechnology Progress, 2008, 24: 214–220

    Article  CAS  Google Scholar 

  30. Markham G D, Pajares M A. Structure-function relationships in methionine adenosyltransferases. Cellular and Molecular Life Sciences, 2009, 66: 636–648

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, Beijing, 100029, China

    Yanhui Liu, Biqiang Chen, Zheng Wang, Luo Liu & Tianwei Tan

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  1. Yanhui Liu
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  2. Biqiang Chen
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  3. Zheng Wang
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  4. Luo Liu
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  5. Tianwei Tan
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Correspondence to Luo Liu or Tianwei Tan.

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Liu, Y., Chen, B., Wang, Z. et al. Functional characterization of a thermostable methionine adenosyltransferase from Thermus thermophilus HB27. Front. Chem. Sci. Eng. 10, 238–244 (2016). https://doi.org/10.1007/s11705-016-1566-2

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