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High-level expression of recombinant IgG1 by CHO K1 platform

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Abstract

The Chinese Hamster Ovary (CHO K1) cell was used to express a targeted anti-cancer monoclonal antibody by optimizing the platform of the construction of production cell line in this study. The adherent CHO K1 was first adapted to suspension culture in chemical defined medium. Then the glutamine synthetase (GS) vector was applied to construct a single plasmid to overexpress a monoclonal antibody IgG1. Post transfection, the production of cell pool was optimized by glutamine-free selection and amplification using various concentrations of methionine sulfoximine. The best cell pool of CHO K1/IgG1 was used to screen the top single clone using the limiting dilution cloning. Finally, a high IgG1 production of 780 mg/L was obtained from a batch culture. This study demonstrated that the construction of high producing cell line, from gene to clone, could be completed within six month and the gene amplification improved protein production greatly.

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Authors and Affiliations

  1. Department of Chemical and Biological Engineering, The University of Alabama, Tuscaloosa, AL, 35294, USA

    Ningning Xu, Jianfa Ou, Al-Karim (Al) Gilani & Margaret Liu

  2. Departments of Medicine and Biomedical Engineering, The University of Alabama at Birmingham, Birmingham, AL, 35294, USA

    Lufang Zhou

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  1. Ningning Xu
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  2. Jianfa Ou
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  3. Al-Karim (Al) Gilani
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  4. Lufang Zhou
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  5. Margaret Liu
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Correspondence to Lufang Zhou.

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Dedicated to the 120th Anniversary of Tianjin University

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Xu, N., Ou, J., Gilani, AK.(. et al. High-level expression of recombinant IgG1 by CHO K1 platform. Front. Chem. Sci. Eng. 9, 376–380 (2015). https://doi.org/10.1007/s11705-015-1531-5

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  • DOI: https://doi.org/10.1007/s11705-015-1531-5

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