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Cryo-copolymerization preparation of dextran-hyaluronate based supermacroporous cryogel scaffolds for tissue engineering applications

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Abstract

Dextran-hyaluronate (Dex-HA) based supermacroporous cryogel scaffolds for soft tissue engineering were prepared by free radical cryo-copolymerization of aqueous solutions containing the dextran methacrylate (Dex-MA) and hyaluronate methacrylate (HA-MA) at various macromonomer concentrations under the freezing condition. It was observed that the suitable total concentration of macromonomers for the preparation of Dex-HA cryogel scaffold with satisfied properties was 5% (w/w) at the HA-MA concentration of 1% (w/v), which was then used to produce the test scaffold. The obtained cryogel scaffold with 5% (w/w) macromonomer solution had high water permeability (5.1 × 10−12 m2) and high porosity (92.4%). The pore diameter examined by scanning electron microscopy (SEM) was in a broad range of 50–135 μm with the mean pore diameter of 91 μm. Furthermore, the cryogel scaffold also had good elastic nature with the elastic modulus of 17.47±1.44 kPa. The culture of 3T3-L1 preadipocyte within the scaffold was investigated and observed by SEM. Cells clustered on the pore walls and grew inside the scaffold indicating the Dex-HA cryogel scaffold could be a promising porous biomaterial for applications in tissue engineering.

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Authors and Affiliations

  1. State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou, 310032, China

    Dongjiao Zhou, Shaochuan Shen, Junxian Yun & Kejian Yao

  2. Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China

    Dong-Qiang Lin

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  1. Dongjiao Zhou
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  2. Shaochuan Shen
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  3. Junxian Yun
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  4. Kejian Yao
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Correspondence to Junxian Yun or Dong-Qiang Lin.

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Zhou, D., Shen, S., Yun, J. et al. Cryo-copolymerization preparation of dextran-hyaluronate based supermacroporous cryogel scaffolds for tissue engineering applications. Front. Chem. Sci. Eng. 6, 339–347 (2012). https://doi.org/10.1007/s11705-012-1209-1

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  • DOI: https://doi.org/10.1007/s11705-012-1209-1

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