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Shoot multiplication of Syringa reticulata var. mandshurica from in vitro cultured seedlings

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Abstract

We developed a shoot multiplication protocol for Syringa reticulata Blume var. mandshurica Hara from in vitro cultured seedlings that derived from in vitro germinated seeds. The shoots could be induced on Murashige and Skoog (MS) medium with proper plant growth regulator combinations of 6-benzylaminopurine (BA) and indole-3-butyric acid (IBA). The better medium for shoot multiplication and growth was MS + 5 mg L−1 BA + 0.5 mg L−1 IBA + 20 g L−1 sucrose + 7 g L−1 agar, and the corresponding shoot induction rate was 75 %. The plantlets grew well after rooting on 1/2MS medium (macro-elements of MS medium are at half-strength) supplemented with 1 mg L−1 IBA, and the survival percentage was >80 % at 16 weeks after transplanting.

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Authors and Affiliations

  1. School of Forestry, Northeast Forestry University, Harbin, 150040, China

    Chun-ping Liu, Ling Yang, Peng Zhang & Hai-long Shen

  2. State Key Laboratory of Forest Genetics and Breeding, Northeast Forestry University, Harbin, 150040, China

    Chun-ping Liu, Bo-wen Yang, Ling Yang, Peng Zhang & Hai-long Shen

  3. Academic Journal Publishing Division, Higher Education Press, Beijing, 100029, China

    Hua-ying Liu

  4. College of Landscape Architecture, Northeast Forestry University, Harbin, 150040, China

    Bo-wen Yang

Authors
  1. Chun-ping Liu
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  2. Hua-ying Liu
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  3. Bo-wen Yang
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  4. Ling Yang
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  5. Peng Zhang
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  6. Hai-long Shen
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Corresponding author

Correspondence to Hai-long Shen.

Additional information

Project funding: This study was supported by the Special Funding for Distinguished Researchers by Northeast Forestry University, China.

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Liu, Cp., Liu, Hy., Yang, Bw. et al. Shoot multiplication of Syringa reticulata var. mandshurica from in vitro cultured seedlings. J. For. Res. 28, 41–46 (2017). https://doi.org/10.1007/s11676-016-0278-2

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  • DOI: https://doi.org/10.1007/s11676-016-0278-2

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