Skip to main content
SpringerLink
Log in

Haoqin Qingdan Decoction (蒿芩清胆汤) and ribavirin therapy downregulate CD14 and toll-like receptor 4 in febrile disease with dampness-heat syndrome in a mouse model

  • Original Article
  • Published:
Chinese Journal of Integrative Medicine Aims and scope Submit manuscript

Abstract

Objective

To evaluate the effect of Chinese medicine Haoqin Qingdan Decoction (蒿芩清胆汤, HQD) for febrile disease dampness-heat syndrome (FDDHS).

Methods

Forty mice were divided into four groups, including normal control, FDDHS (induced by Radix et Rhizoma Rhei recipe and influenza virus A1 FM1 model), HQD, and the ribavirin groups (10 in each). The normal control and FDDHS groups were administered normal saline. HQD and the ribavirin groups were administered HQD and ribavirin intragastrically once daily at a dose of 64 g/(kg d) and 0.07 g/(kg d), respectively for 7 days. Lethargy, rough hair, diarrhea, tongue color and sole color were evaluated for pathological changes in morphology. The tongue and lung tissues were collected for histology. The CD14 and toll-like receptor 4 (TLR4) expression levels were measured using real-time quantitative polymerase chain reaction.

Results

More than 80% of the FDDHS mice showed hypokinesia and lethargy, and pathological changes associated with rough hair, diarrhea, tongue color and sole color. With advanced treatment for 7 days, the thick greasy tongue fur of the HQD and ribavirin groups were thinner than that of the FDDHS group (P<0.05), and it was the thinnest in the ribavirin group as compared with that in other groups (P<0.05). The CD14 and TLR4 expression levels in the lung tissues of HQD and ribavirin groups significantly delined compared with the model group (P<0.05 or P<0.01). CD14 was down-regulated more remarkably in the HQD group compared with the ribavirin group (P<0.05), whereas the converse was true with TLR4 (P<0.05).

Conclusions

We established a FDDHS mouse model showing systemic clinical symptoms. Both HQD and ribavirin can inhibit the expression of CD14 and TLR4 in FDDHS mice, while the effect of ribavirin might be much more violent. The expression changes of CD14 and TLR4 consistently refers to lipopolysaccharide, the commonly and hotly inducing factor in FDDHS.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ren X, Wen X, Hong B, Liao Y, Ma W, Tang Y. Effects of Sanren Decoction on Th1/Th2 cytokines in rats with spleenstomach damp-heat syndrome. J South Med Univ (Chin) 2012;32:181–184.

    Google Scholar 

  2. Wang ZZ, Fang YF, Wang Y, Mu FX, Chen J, Zou QH, et al. Logistic regression analysis of damp-heat and cold-damp impeding syndrome of rheumatoid arthritis: a perspective in Chinese medicine. Chin J Integr Med 2012;18:575–581.

    Article  PubMed  Google Scholar 

  3. He HH, Shen H, Zheng K. Observation of the curative effect of Qingchang Huashi Recipe for treating active ulcerative colitis of inner-accumulation of damp-heat syndrome. Chin J Integr Tradit West Med (Chin) 2012;32:1598–1601.

    Google Scholar 

  4. Yang CJ, Liu HW, Wang LC. Study on the differential gene expressions of chronic hepatitis B patients of Gan depression Pi deficiency syndrome and Pi-Wei damp-heat syndrome. Chin J Integr Tradit West Med (Chin) 2012;32:1032–1037.

    CAS  Google Scholar 

  5. Zhang SJ, Chen Z, Li GW, BL Wang. Effect of the Haoqinqingdan Decoction on damp-heat syndrome in rats with influenza viral pneumonia. Asian Pac J Trop Med 2013;6:653–657.

    Article  PubMed  Google Scholar 

  6. Ji G, Fan JG, Chen JJ, Lu LG, Xing LJ, Zheng PY, et al. Effectiveness of Danning Tablet in patients with nonalcoholic fatty liver of damp-heat syndrome type: a multicenter randomized controlled trial. J Chin Integr Med 2008;6:128–133.

    Article  Google Scholar 

  7. Pan Y, Luo BD, Lin PZ, Liu Y, Wan WR, Guo JQ. The mechanism of signal extension in Haoqin Qingdan Decoction immunity activity in damp-heat syndrome of pneumonia disease infected by influenza virus. Chin J Prevent Med (Chin) 2010;44:612–616.

    Google Scholar 

  8. Prescott JB, Hall PR, Bondu-Hawkins VS, Ye C, B Hjelle. Early innate immune responses to Sin Nombre hantavirus occur independently of IFN regulatory factor 3, characterized pattern recognition receptors, and viral entry. J Immunol 2007;179:1796–1802.

    Article  CAS  PubMed  Google Scholar 

  9. Zanoni I, Granucci F. Role of CD14 in host protection against infections and in metabolism regulation. Front Cell Infect Microbiol 2013;3:32.

    Article  PubMed  PubMed Central  Google Scholar 

  10. Zanoni I, Ostuni R, Marek LR, Barresi S, Barbalat R, Barton GM, et al. CD14 controls the LPS-induced endocytosis of toll-like receptor 4. Cell 2011;147:868–880.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Kim D, Kim JY. Anti-CD14 antibody reduces LPS responsiveness via TLR4 internalization in human monocytes. Mol Immunol 2013;57:210–215.

    Article  PubMed  Google Scholar 

  12. Shirey KA, Lai W, Scott AJ, Lipsky M, Mistry P, Pletneva LM, et al. The TLR4 antagonist Eritoran protects mice from lethal influenza infection. Nature 2013;497:498–502.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Imai Y, Kuba K, Neely GG, Yaghubian-Malhami R, Perkmann T, van Loo G, et al. Identification of oxidative stress and toll-like receptor 4 signaling as a key pathway of acute lung injury. Cell 2008;133:235–249.

    Article  CAS  PubMed  Google Scholar 

  14. He LQ. Application of the theory of damp-heat in Spleen and Stomach in treatment of kidney diseases. J Chin Integr Med (Chin) 2004;2:7–9.

    Article  Google Scholar 

  15. Zhang SF, Ling CQ, Li B, Chen HY, Chen Z. Effects of Sisheng Decoction on the immunity and anti-stress function in mice with Spleen deficiency syndrome. J Chin Integr Med 2012;10:1465–1469.

    Article  Google Scholar 

  16. Liang R, Li Y, Wang SH. Analysis on tongue manifestations of 488 cases of Warm disease in Qing Dynasty. Chin J Med History (Chin) 2006;36:131–134.

    Google Scholar 

  17. Xu Y, Zeng CC, Cai XY, Guo RP, Nie G, Jin Y. Chromaticity and optical spectrum colorimetry of the tongue color in different syndromes of primary hepatic carcinoma. J Chin Integr Med (Chin) 2012;10:1263–1271.

    Article  Google Scholar 

  18. Chen Q. Experiences in treatment based on syndrome differentiation for black fur caused by dampness stagnancy due to Spleen deficiency. J Chin Integr Med (Chin) 2006;4:320.

    Article  Google Scholar 

  19. Zhuo YY, Yang ZX, Wu JM. Effect of electroacupuncture on differentiation and proliferation of hippocampal nerve stem cells in splenic asthenia pedo-rats. Acupunc Res (Chin) 2011;36:327–334.

    Google Scholar 

  20. Shi JH, Ding H. Effects of hygrothermal environment on endotoxin induced newzealand rabbit’s liver, colon tumor necrosis factor expression. J Shaanxi Coll Tradit Chin Med (Chin) 2014;37:62–65.

    Google Scholar 

  21. Jue TS, Chang LP, Lv JY, Li SG, Huang LP, Huang P, et al. Effect of modified Huopu Xialing Decoction on TNF-α, IL-10 and blood lipid metabolism of rats with febrile disease damp-heat syndrome (FDDHS). J Guangxi Med Univ (Chin) 2012;29:197–200.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Journal-Publishing Center, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China

    Huan-huan Luo  (骆欢欢)

  2. Department of Tropical Medicine, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China

    Feng-xue Zhang  (张奉学)

  3. Dean’s Office, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, 510405, China

    Wei Wu  (吴 薇)

  4. Headmaster’s Office, Guangzhou Medical University, Guangzhou, 510182, China

    Xin-hua Wang  (王新华)

Authors
  1. Huan-huan Luo  (骆欢欢)
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Feng-xue Zhang  (张奉学)
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wei Wu  (吴 薇)
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xin-hua Wang  (王新华)
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xin-hua Wang  (王新华).

Additional information

Supported by the National Natural Science Foundation for Distinguished Young Scholars of China (No. 81102535)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Luo, Hh., Zhang, Fx., Wu, W. et al. Haoqin Qingdan Decoction (蒿芩清胆汤) and ribavirin therapy downregulate CD14 and toll-like receptor 4 in febrile disease with dampness-heat syndrome in a mouse model. Chin. J. Integr. Med. 22, 768–773 (2016). https://doi.org/10.1007/s11655-016-2097-2

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11655-016-2097-2

Keywords

Search

Navigation