Abstract
Objective
To examine whether ginsenoside Rg1 (Rg1) inhibits the high-voltage-activated calcium currents (ICa,HVA) via mitogen-activated protein kinase (MAPK) in hippocampal neurons in rat brain slices exposed to beta-amyloid peptide 25–35 (Aβ25–35).
Methods
An experimental Alzheimer disease (AD) model was prepared by exposure of rat brain slices to Aβ25–35 (10 µmol/L). After treatment with Rg1 (20 µmol/L), the ICa,HVA elicited in hippocampal neurons in these rat brain slices upon depolarization from–40 to 40 mV for 200 ms was recorded by a whole-cell patch clamp to analyze the changes in the peak current density, I–V curve, activation-V curve, and inactivation-V curve.
Results
Exposure of rat brain slices to Aβ led to a significant increase in ICa,HVA, enhancement of the voltage sensitivity of channel activation, and reduction of the voltage sensitivity of channel inactivation in neurons in the hippocampus of rat brain slices. Rg1 treatment significantly inhibited these changes. These effects of Rg1 could be effectively inhibited by the MAPK inhibitor PD98059.
Conclusion
Rg1 can inhibit Ica,HVA via MAPK in hippocampal neurons in Aβ-exposed rat brain slices.
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Supported by the Tackle Key Problems in Science and Technology Foundation of Shaanxi Province, China [No. 2007K16-07(5)]; and the Program of Administration of Traditional Chinese Medicine of Shaanxi Province, China (No. 2005030)
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Quan, Qk., Li, X., Yuan, Hf. et al. Ginsenoside Rg1 inhibits high-voltage-activated calcium channel currents in hippocampal neurons of beta-amyloid peptide-exposed rat brain slices. Chin. J. Integr. Med. (2016). https://doi.org/10.1007/s11655-015-2301-4
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DOI: https://doi.org/10.1007/s11655-015-2301-4