Abstract
Objective
To investigate the effect of hydro-ethanolic extract of Zataria multiflora (Z. multiflora) on endothelin level, total and differential white blood cells (WBC) count of sensitized guinea pigs.
Methods
Five groups of guinea pigs sensitized to ovalbumin (OA) were given drinking water alone (group S), drinking water containing three concentrations of Z. multiflora (0.2, 0.4 and 0.8 mg/mL as groups S+Z1, S+Z2 and S+Z3) and dexamethasone (group S+D), n=6 for each group. The endothelin levels as well as total and differential WBC count in blood of sensitized and control guinea pigs were evaluated using enzyme linked immunosorbent assay method, and hemocytometer and Wright-Giemsa's staining of blood sample smear respectively.
Results
Blood endothelin levels, total and most differential WBC count were increased but lymphocytes decreased in sensitized animals compared to controls (allP <0.01). In groups S+D, S+Z2 and S+Z3 endothelin level, total and differential WBC counts were significantly improved compared with group S (P <0.01). Although, all measured parameters in group S+Z1 was lower than group S+D (P <0.01), some parameters in group S+Z3 were greater than in group S+D (P <0.05 toP <0.01).
Conclusion
The results showed an anti-inflammatory effect of Z. multiflora extract in sensitized guinea pigs, which may suggest a therapeutic potential for the plant on asthma.
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Acknowledgements
The results described in this paper were part of Sediqeh Jalali's Ph.D. student thesis.
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Boskabady MH designed the study, supervised experiments, helped in statistical analysis and manuscript preparation, Jalalis perform experiments, helped in statistical analysis and manuscript preparation.
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There is no conflict of interest in the present study.
Supported by Research Department of Mashhad University of Medical Sciences (No. 89431)
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Boskabady, M.H., Jalali, S. Effect of Zataria multiflora Extract on Total and Differential White Blood Cell Count and Endothelin Level in Blood of Ovalbumin Sensitized Guinea Pigs. Chin. J. Integr. Med. 26, 604–608 (2020). https://doi.org/10.1007/s11655-015-2129-y
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DOI: https://doi.org/10.1007/s11655-015-2129-y