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Differential expression of microRNA in endothelial cells incubated with serum of hypertension patients with blood-stasis syndrome

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Abstract

Objective

To screen out blood-stasis syndrome (BSS)-associated microRNA and therefore determine the possible target for treating hypertension.

Methods

A high-energy sequencing method and digital gene expression sequencing theory were adopted to sequence microRNA (miRNA) and messenger RNA (mRNA), and to determine differential expression in human umbilical vein endothelial cells incubated with serum samples from hypertension patients with or without BSS, and healthy controls. The results were confifirmed using gene prediction software.

Results

A total of 13 miRNAs and 11 mRNAs showed statistical difference both in the BSS/normal groups and BSS/non-BSS groups, respectively. Four pairs of target mRNA/miRNA were identifified: FRMD4A/hsa-miR-34a, MAP3K14/hsa-miR-34a, PER1/hsa-miR-34a, and FGF2/hsa-miR-132.

Conclusion

Four mRNA/miRNA pairs mentioned above seem to be involved in pathogenesis and maintenance of hypertension with BSS.

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Authors and Affiliations

  1. Faculty of Traditional Chinese Medicine, Medical School of Jinan University, Guangzhou, 510632, China

    Ming-ming Wang  (王铭铭), Mei-xia Fang  (方梅霞), Li-guo Chen  (陈利国), Hua-qiang Wang  (王华强), Hong-jie Liu  (刘红杰) & Hai-lan Tang  (唐海兰)

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  1. Ming-ming Wang  (王铭铭)
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  2. Mei-xia Fang  (方梅霞)
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  3. Li-guo Chen  (陈利国)
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  4. Hua-qiang Wang  (王华强)
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  5. Hong-jie Liu  (刘红杰)
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  6. Hai-lan Tang  (唐海兰)
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Correspondence to Li-guo Chen  (陈利国).

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Wang, Mm., Fang, Mx., Chen, Lg. et al. Differential expression of microRNA in endothelial cells incubated with serum of hypertension patients with blood-stasis syndrome. Chin. J. Integr. Med. 21, 817–822 (2015). https://doi.org/10.1007/s11655-015-2053-1

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  • DOI: https://doi.org/10.1007/s11655-015-2053-1

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