Abstract
Objective
To observe the morphological changes in enteric nerve system (ENS) of rats with multiple organ dysfunction syndrome (MODS) treated by Dachengqi Decoction (大承气汤, DCQD).
Methods
Fifty Wistar rats were randomly assigned to the control group, MODS model group and DCQD treated group. The rats in MODS model group and DCQD treated group were injected Escherichia coli (E. coli) suspension into abdominal cavity under sterile condition. The DCQD treated group was gavaged with DCQD 2 days before the E. coli suspension was injected. Twenty-four hours after injection, the proximal segment of intestine was resected and studied by immunohistofluorescence using vesicular acetylcholine transporter, vasoactive intestinal polypeptide (VIP), substance P (SP) and neuronal nitric oxide synthase (nNOS) antibodies. The whole-mount preparations were observed by laser scanning confocal microscope to detect the changes of quantity and fluorescence integral optical density (IOD) value of intestine enteric nerves.
Results
Compared with the control group, the quantity and IOD value of acetylcholine (ACh), VIP, SP and nitric oxide (NO) nerves of intestine in the MODS group were significantly decreased (P<0.01), and the network of enteric nerves was remarkably disrupted. Compared with the MODS group, the quantity and fluorescence IOD value of ACh, VIP, SP and NO nerves in the DCQD group were significantly increased (P<0.01), and the network of enteric nerves was remarkably recovered.
Conclusion
DCQD can protect and repair damage in the network of ACh, SP, NO and VIP nerves in rats with MODS, which may be one of mechanisms involved in promoting gastrointestinal motility by DCQD.
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References
Matějovic M, Rokyta R Jr, Krouzecký A, Novák I. Gasrtointestinal tract dysfunction in critical illness. Gas Lek Cesk 2002;141(2):46–50.
Bao YG, Shu XL, Li XB, Gu WZ, Ying AJ, Zhao C, et al. Roles of enteric nervous system neurotransmitters and interstitial cells of Cajal in the colon in slow transit constipation in rats. Chin J Contemporary Pediatrics (Chin) 2009;11:481–485.
Mitolo-Chieppa D, Mansi G, Rinaldi R, Montagnani M, Potenza MA, Genualdo M, et al. Cholinergic stimulation and nonadrenergic, noncholinergic relaxation of human colonic circular muscle in idiopathic chronic constipation. Dig Dis Sci 1998;43:2719–2726.
Chen ZY, Qi QH. Changes of colon motility and mechanism in rats with multiple organ dysfunction syndrome. Chin J Exp Surg (Chin) ia]5._The Ministry of Science and Technology of the People’s Republic of China. Guidance Suggestions for the Care and Use of Laboratory Animals. 2006-09-30.
Brehmer A, Rupprecht H, Neuhuber W. Two submucosal nerve plexus in human intestines. Histochem Cell Biol 2010;133:149–161.
Bai YH, Zhang J, Shi HY, Dai F, Li FW. Expression of metabotropic glutamate receptors mGluR1 and mGluR5 in the myenteric plexus of the diabetic rat ileum. World Chin J Digestol 2008;16:3734–3739.
Tian ZS, Wu XZ, Chen C. Point of view about abdomen brain (second brain). Chin J Integr Tradit West Med (Chin) 2005;11:454–457.
Sri Paran T, Rolle U, Puri P. Developmental changes of the adrenergic network in the myenteric plexus of the porcine small bowel. Pediatr Surg Int 2007;23:659–663.
Burns AJ, Pachnis V. Development of the enteric nervous system: bringing together cells, signals and genes. Neurogastroenterol Moti 2009;21:100–102.
Porter AJ, Wattchow DA, Brookes SJ, Costa M. Cholinergic and nitrergic interneurones in the myenteric plexus of the human co1on. Gut 2002;51:70–75.
Shah V, Lyford G, Gores G, Farrugia G. Nitric oxide in gastrointestinal health and disease. Gastroenterology 2004;126:903–913.
Hens J, Gajda M, Scheuermann DW, Adriaensen D, Timmermans JP. The longitudinal smooth muscle layer of the pig small intestine is innervated by both myenteric and submucous neurons. Histochem Cell Biol 2002;117:481–492.
Vulchanova L, Olson TH, Stone LS, Riedl MS, Elde R, Honda CN. Cytotoxic targeting of isolectin IB4-binding sensory neurons. Neuroscience 2001;108:143–155.
Milner P, Crowe R, Kamm MA, Lennard-Jones JE, Burnstock G. Vasoactive intestinal polypeptide levels in sigmoid colon in idiopathic constipation and diverticular disease. Gastroenterology 1990;99:666–675.
Stead RH, Tomioka M, Quinonez G, Simon GT, Felten SY, Bienenstock J. Intestinal mucosal mast cells in normal and nematode-infected rat intestines are in intimate contact with peptidergic nerves. Proc Natl Acad Sei USA 1987;84:2975–2979.
Wang LH, Fang XC, Pan GZ. Bacillary dysentery as a causative factor of irritable bowel syndrome and its pathogenesis. Gut 2004;53:1096–1101.
Törnblom H, Lindberg G, Nyberg B, Veress B. Full-thickness biopsy of the jejunum reveals inflammation and enteric neuropathy in irritable bowel syndrome. Gastroenterology 2002;123:1972–1979.
D’Andrea MR, Saban MR, Gerard NP, Wershil BK, Saban R. Lack of neurokinin-1 receptor expression affects tissue mast cell numbers but not their spatial relationship with nerves. Am J Physiol Regul Integr Comp Physiol 2005;288:491–500.
De Jonge F, De Laet A, Van Nassauw L, Brown JK, Miller HR, van Bogaert PP, et al. In vitro activation of murine DRG neurons by CGRP-mediated mucosal mast cell degranulation. Am J Physiol Gastrointest Liver Physiol 2004;287:178–191.
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Supported by the National Natural Science Foundation of China (No. 30973852 and No. 30772860)
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Xie, Mz., Qi, Qh., Zhang, Sl. et al. Effects of Dachengqi Decoction (大承气汤) on morphological changes in enteric nerve system of rats with multiple organ dysfunction syndrome. Chin. J. Integr. Med. 21, 624–629 (2015). https://doi.org/10.1007/s11655-015-1978-8
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DOI: https://doi.org/10.1007/s11655-015-1978-8