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Differential responses of Mimusops elengi and Manilkara zapota seeds and embryos to cryopreservation

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Abstract

Several dehydration protocols were evaluated for their ability to cryopreserve intact seeds and excised embryonic axes of Mimusops elengi and Manilkara zapota (Sapotaceae). Both interspecific and intraspecific variations in cryotolerance were found. M. zapota embryonic axes were more tolerant of cryopreservation than those of M. elengi, and showed higher desiccation tolerance, higher post-thawing survival and development, and a much wider range of moisture contents for cryopreservation. Maximum development rates were 94% and 27% for M. zapota and M. elengi, respectively. Intact seeds of both species tolerated desiccation to low moisture levels, but were sensitive to liquid nitrogen exposure, and cryopreserved seeds failed to germinate. Assessment of developing embryos excised from cryopreserved seeds associated nonviability of cotyledons and plumules with germination failure. Other structures survived at variable rates; most hypocotyls and radicles (up to 76% and 98% for M. elengi and M. zapota, respectively) were viable. The different cryotolerance between hypocotyls and cotyledons is a critical cause for failure in cryopreservation, contributing to the difficulty in developing protocols for such intermediate oily seeds.

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Acknowledgments

We are grateful to Prof. Richard Corlett and Dr. Uromi Manage Goodale in our botanical garden for providing language help and the National Natural Science Foundation of China (31170626) and National Science and Technology Support Program (2012BAC01B05) for providing financial support for this research.

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Authors and Affiliations

  1. Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla, Yunnan, 666303, China

    Bin Wen, Xiaofeng Wang & Yunhong Tan

  2. Group of Seed Physiology and Biotechnology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China

    Songquan Song

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  1. Bin Wen
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  2. Xiaofeng Wang
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  3. Yunhong Tan
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  4. Songquan Song
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Correspondence to Bin Wen.

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Editor: Rida Shibli

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Wen, B., Wang, X., Tan, Y. et al. Differential responses of Mimusops elengi and Manilkara zapota seeds and embryos to cryopreservation. In Vitro Cell.Dev.Biol.-Plant 49, 717–723 (2013). https://doi.org/10.1007/s11627-013-9562-4

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