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In vitro conservation of chestnut (Castanea sativa) by slow growth

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Abstract

Slow growth storage has been achieved for Castanea sativa (cv. ‘Montemarano’) shoot cultures over a duration of 48 mo at a temperature of 8°C, where 82% of explants survived and were able to resume normal growth after transfer to standard culture conditions at 23°C. The evaluation of the chlorophyll content of leaves also showed no differences between material stored for 48 mo and control material subcultured at 23°C. With a storage temperature of 4°C, the survival of shoots was significantly lower at approximately 56% after 12 mo, and no plants recovered after 24-mo storage. The presence of 6-benzyladenine 0.44 μM in the culture medium proved to be necessary for the recovery of healthy shoots, while pre-treatments with different concentrations of abscisic acid did not significantly influence the survival of shoots following storage conditions. A low level of light during slow growth storage resulted in positive effects on the rate of shoot survival over the longest preservation periods.

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Acknowledgments

We wish to sincerely thank Dr. Matthew Haworth from Institute of Biometeorology of the National Research Council (IBIMET-CNR) for his valuable help in the correction of the English manuscript. We are also grateful to Mrs. Catia Boggi and Mr. Gabriele Cencetti for their technical support.

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Authors and Affiliations

  1. Istituto di Genetica Vegetale (IGV-CNR), via Madonna del Piano 10, 50019, Sesto Fiorentino, FI, Italy

    Maurizio Capuana

  2. Istituto di Biometeorologia (IBIMET-CNR), via Giovanni Caproni 8, 50145, Florence, Italy

    Sara Di Lonardo

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  1. Maurizio Capuana
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  2. Sara Di Lonardo
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Correspondence to Maurizio Capuana.

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Editor: J. Forster

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Capuana, M., Di Lonardo, S. In vitro conservation of chestnut (Castanea sativa) by slow growth. In Vitro Cell.Dev.Biol.-Plant 49, 605–610 (2013). https://doi.org/10.1007/s11627-013-9536-6

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  • DOI: https://doi.org/10.1007/s11627-013-9536-6

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