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Characterization of embryonic stem cell model of polycystic ovary syndrome

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Abstract

The aims of this study are to establish an embryonic stem (ES) cell model of polycystic ovary syndrome and to characterize this ES cell line. ES cells were isolated and cultured from 322 wasted fertilized embryos from polycystic ovary syndrome (PCOS) patients in vitro. They were also characterized by development and differential markers. ES cells from PCOS subject present normal development profile with ES-specific markers such as OCT-4 and SSEA-4. These ES cells can also differentiate into three germ layer derivatives and form teratomas in vivo. ES cells from PCOS patients pose development and differentiation potentials as you would expect of cells from non-PCOS patients; therefore, they can be used as a cellular model to study the pathology of PCOS.

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Authors and Affiliations

  1. Department of Gynecology and Obstetrics, Tianjin Medical University General Hospital, Tianjin, 300052, China

    Yue Zhang & Feng-xia Xue

  2. Tianjin Central Hospital of Gynecology Obstetrics, Tianjin, 300052, China

    Yue Zhang & Yun-shan Zhang

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  1. Yue Zhang
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  2. Yun-shan Zhang
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  3. Feng-xia Xue
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Correspondence to Feng-xia Xue.

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Editor: J. Denry Sato

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Zhang, Y., Zhang, Ys. & Xue, Fx. Characterization of embryonic stem cell model of polycystic ovary syndrome. In Vitro Cell.Dev.Biol.-Animal 52, 507–511 (2016). https://doi.org/10.1007/s11626-016-0040-2

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  • DOI: https://doi.org/10.1007/s11626-016-0040-2

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