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Chondroitin sulfate effects on neural stem cell differentiation

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Abstract

We have investigated the role chondroitin sulfate has on cell interactions during neural plate formation in the early chick embryo. Using tissue culture isolates from the prospective neural plate, we have measured neural gene expression profiles associated with neural stem cell differentiation. Removal of chondroitin sulfate from stage 4 neural plate tissue leads to altered associations of N-cadherin-positive neural progenitors and causes changes in the normal sequence of neural marker gene expression. Absence of chondroitin sulfate in the neural plate leads to reduced Sox2 expression and is accompanied by an increase in the expression of anterior markers of neural regionalization. Results obtained in this study suggest that the presence of chondroitin sulfate in the anterior chick embryo is instrumental in maintaining cells in the neural precursor state.

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Acknowledgments

We greatly appreciate the provision of probe DNA from the labs of Diana Darnell, Paola Bovelenta, and Michael Kessel. Christian Clark and Satinder Sidhu contributed technical assistance.

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Authors and Affiliations

  1. Department of Biological Sciences, Murray State University, Murray, KY, 42071, USA

    David R. Canning, Natalie R. Brelsford & Neil W. Lovett

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  1. David R. Canning
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  2. Natalie R. Brelsford
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  3. Neil W. Lovett
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Correspondence to David R. Canning.

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Editor: Tetsuji Okamoto

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Canning, D.R., Brelsford, N.R. & Lovett, N.W. Chondroitin sulfate effects on neural stem cell differentiation. In Vitro Cell.Dev.Biol.-Animal 52, 35–44 (2016). https://doi.org/10.1007/s11626-015-9941-8

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