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Myo10 is required for neurogenic cell adhesion and migration

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Abstract

Myosin X (Myo10), an untraditional member of myosin superfamily, is characterized as an actin-based molecular motor, which plays a critical role in diverse cellular motile events. Previous research by our group has found that Myo10 influenced neuronal radial migration in developing neocortex, but the underlying mechanism is still largely unknown. In this study, we found that knockdown of endogenous Myo10 in a normal gonadotropin-releasing hormone (GnRH) neuronal cell line transfected with the large T antigen (NLT) induced the impairment of cell motility and orientation. In the wound healing assay, with the Golgi complex staining to display cell polarity, Myo10 knockdown cells were randomly oriented compared to the control. Furthermore, suppressing the expression of Myo10 decreased the ability of cell–matrix adhesion. N-cadherin, a calcium-dependent classical cell adhesion molecule, rescued the migration deficiency caused by Myo10 knockdown in cell aggregates and collagen gel assay. These results suggest that Myo10 is required for neurogenic cell migration through N-cadherin mediated cell adhesion.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31271486, 31171042, 31401192), the Fundamental Research Funds for the Central Universities (12QNJJ018), the Jilin Province Science Foundation for Youths (20130522004JH), and the Jilin postdoctoral fund and the Natural Science Foundation of Changchun Normal University (202-02814).

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Authors and Affiliations

  1. Key laboratory of Molecular Epigenetics of Ministry of Education, Institute of Cytology and Genetics, Northeast Normal University, No. 5268, Renmin Street, Changchun, Jilin, 130024, People’s Republic of China

    Huali Yu, Yuguang Guo, Lin Yuan, Xingzhi Wang & Xiaojuan Zhu

  2. Institute of Basic Medicine, Dali University, Dali, 671000, Yunnan, People’s Republic of China

    Mingming Lai

  3. School of Physical Education, Changchun Normal University, Changchun, 130032, Jilin, People’s Republic of China

    Yongsheng Lan

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  1. Huali Yu
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  2. Mingming Lai
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  3. Yuguang Guo
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  4. Lin Yuan
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  5. Yongsheng Lan
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  6. Xingzhi Wang
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  7. Xiaojuan Zhu
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Corresponding author

Correspondence to Xiaojuan Zhu.

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Editor: T. Okamoto

Huali Yu and Mingming Lai contributed equally to this work.

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Yu, H., Lai, M., Guo, Y. et al. Myo10 is required for neurogenic cell adhesion and migration. In Vitro Cell.Dev.Biol.-Animal 51, 400–407 (2015). https://doi.org/10.1007/s11626-014-9845-z

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  • DOI: https://doi.org/10.1007/s11626-014-9845-z

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