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Expression of Transthyretin during bovine myogenic satellite cell differentiation

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Abstract

Adult myogenesis responsible for the maintenance and repair of muscle tissue is mainly under the control of myogenic regulatory factors (MRFs) and a few other genes. Transthyretin gene (TTR), codes for a carrier protein for thyroxin (T4) and retinol binding protein bound with retinol in blood plasma, plays a critical role during the early stages of myogenesis. Herein, we investigated the relationship of TTR with other muscle-specific genes and report their expression in muscle satellite cells (MSCs), and increased messenger RNA (mRNA) and protein expression of TTR during MSCs differentiation. Silencing of TTR resulted in decreased myotube formation and decreased expression of myosin light chain (MYL2), myosin heavy chain 3 (MYH3), matrix gla protein (MGP), and voltage-dependent L type calcium channel (Cav1.1) genes. Increased mRNA expression observed in TTR and other myogenic genes with the addition of T4 decreased significantly following TTR knockdown, indicating the critical role of TTR in T4 transportation. Similarly, decreased expression of MGP and Cav1.1 following TTR knockdown signifies the dual role of TTR in controlling muscle myogenesis via regulation of T4 and calcium channel. Our computational and experimental evidences indicate that TTR has a relationship with MRFs and may act on calcium channel and related genes.

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Acknowledgments

This work was supported by a grant from the BioGreen 21 Program (Project No. PJ907099), Rural Development Administration, Republic of Korea. All research materials used in this study were provided by the Bovine Genome Resources Bank, Yeungnam University, Gyeongsan, Korea.

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Authors and Affiliations

  1. School of Biotechnology, Yeungnam University, Gyeongsan, 712-749, Republic of Korea

    Smritee Pokharel, Majid Rasool Kamli, Bilal Ahmad Mir, Adeel Malik, Eun Ju Lee & Inho Choi

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  1. Smritee Pokharel
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  2. Majid Rasool Kamli
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  3. Bilal Ahmad Mir
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  4. Adeel Malik
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  5. Eun Ju Lee
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Correspondence to Eun Ju Lee or Inho Choi.

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Editor: T. Okamoto

Smritee Pokharel and Majid Rasool Kamli contributed equally to this work

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Supplementary Figure 1

A) Timecourse study on MyoD on MSCs cultured for 10, 12,14, 16 and 18 days. B) T4 treatment and its effect on TTR mRNA expression at day 16. (TIFF 1.25 mb)

Fig6

A) Timecourse study on MyoD on MSCs cultured for 10, 12,14, 16 and 18 days. B) T4 treatment and its effect on TTR mRNA expression at day 16. (GIF 75.9 mb)

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Pokharel, S., Kamli, M.R., Mir, B.A. et al. Expression of Transthyretin during bovine myogenic satellite cell differentiation. In Vitro Cell.Dev.Biol.-Animal 50, 756–765 (2014). https://doi.org/10.1007/s11626-014-9757-y

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