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BMP signaling regulates the differentiation of mouse embryonic stem cells into lung epithelial cell lineages

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Abstract

Somatic stem/progenitor cells are known to be present in most adult tissues. However, those in the lung have limited abilities for tissue regeneration after serious damage as a result of chronic disease. Therefore, regenerative medicine using exogenous stem cells has been suggested for the treatment of progressive lung diseases such as chronic obstructive pulmonary disease and pulmonary fibrosis. Embryonic stem (ES) cells and induced pluripotent stem cells, with their potent differentiation abilities, are promising sources for the generation of various tissue cells. In this study, we investigated the effects of various differentiation-inducing growth factors on the differentiation of lung cells from ES cells in vitro. Several factors, including activin, nodal, and noggin, significantly promoted the induction of Nkx2.1-positive lung progenitor cells when cells were cultured as embryoid bodies. Bone morphogenetic protein (BMP) 4 signaling controls the lineage commitment of lung cells along the proximal–distal axis. BMP4 promotes the induction of distal cell lineages of alveolar bud, such as Clara cells and mucus-producing goblet cells. These results suggest that several developmentally essential factors, including nodal/activin and BMP signaling, are important in the control of the differentiation of lung epithelial cells from mouse ES cells in vitro.

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Acknowledgments

We would like to thank Dr. Shinji Masui and Dr. Hitoshi Niwa for providing us with the plasmids and mouse ES cells. This work was supported in part by Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (M.A. and A.K.), grants from Ichiro Kanahara Foundation (A.K.), and grants from the Suzuken Memorial Foundation (A.K.).

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Authors and Affiliations

  1. Department of Life Sciences (Biology), Graduate School of Arts and Sciences, The University of Tokyo, Meguro, Tokyo, Japan

    Naoto Ninomiya, Tatsuo Michiue & Makoto Asashima

  2. Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan

    Makoto Asashima & Akira Kurisaki

  3. Graduate School of Life and Environmental Sciences, The University of Tsukuba, Tsukuba, Ibaraki, Japan

    Akira Kurisaki

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  1. Naoto Ninomiya
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  2. Tatsuo Michiue
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  3. Makoto Asashima
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  4. Akira Kurisaki
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Correspondence to Akira Kurisaki.

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Editor: T. Okamoto

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Ninomiya, N., Michiue, T., Asashima, M. et al. BMP signaling regulates the differentiation of mouse embryonic stem cells into lung epithelial cell lineages. In Vitro Cell.Dev.Biol.-Animal 49, 230–237 (2013). https://doi.org/10.1007/s11626-013-9589-1

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  • DOI: https://doi.org/10.1007/s11626-013-9589-1

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