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Molecular cloning and characterization of porcine indoleamine 2, 3-dioxygenase and its expression in various tissues

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Summary

In order to confirm the existence of indoleamine 2, 3-dioxygenase (IDO) gene in swine, and to clone the novel gene followed by the molecule structure properties and expression pattern analysis, the porcine mRNA sequences homologous to human IDO were obtained from GenBank database by bioinformatics method. By using RT-PCR, the IDO gene was cloned from porcine endothelial cell line and the accuracy of the nucleic acid sequence was confirmed, and the expression pattern of the gene was detected. The three-dimensional structure model of porcine IDO was built referring to the tertiary structure of human IDO using biological sequence analysis software and database. The results showed that the porcine IDO was identified by sequencing. The nucleotide sequences were confirmed as a novel gene after submitted to Genbank. Porcine IDO was expressed in the lung, thymus, epididymis and anterior chamber with a basic level, however in peripheral blood mononuclear cells (PBMCs) the IDO gene was highly expressed. The three-dimensional structure model of porcine IDO was similar to that of human IDO. It was suggested that identification of the structure information of porcine IDO is essential to further investigate the immunologic function of the gene. Study of IDO on NK cells-mediated xenograft rejection will be a novel therapeutic target for the development of xenotransplantation.

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Authors and Affiliations

  1. Department of Anesthesiology, Huazhong University of Science and Technology, Wuhan, 430030, China

    Chao Chen  (陈 超)

  2. Department of Pediatric Surgery, Huazhong University of Science and Technology, Wuhan, 430030, China

    Mingfa Wei  (魏明发)

  3. Institute of Organ Transplantation, Huazhong University of Science and Technology, Wuhan, 430030, China

    Lu Wang  (王 璐) & Ying Xiang  (向 莹)

  4. Department of General Thoracic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Xiangning Fu  (付向宁) & Min Zhu  (朱 珉)

  5. Key Laboratory of Organ Transplantation, Ministry of Education, Wuhan, 430030, China

    Lu Wang  (王 璐) & Ying Xiang  (向 莹)

  6. Key Laboratory of Organ Transplantation, Ministry of Health, Wuhan, 430030, PR China

    Lu Wang  (王 璐) & Ying Xiang  (向 莹)

Authors
  1. Chao Chen  (陈 超)
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  2. Mingfa Wei  (魏明发)
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  3. Lu Wang  (王 璐)
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  4. Ying Xiang  (向 莹)
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  5. Xiangning Fu  (付向宁)
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  6. Min Zhu  (朱 珉)
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Corresponding author

Correspondence to Min Zhu  (朱 珉).

Additional information

This project was supported by grants from Wuhan Youth Chenguang Project (No. 201050231077) and National Natural Science Foundation of China (No. 30600571 and No. 81172786).

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Chen, C., Wei, M., Wang, L. et al. Molecular cloning and characterization of porcine indoleamine 2, 3-dioxygenase and its expression in various tissues. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 32, 473–479 (2012). https://doi.org/10.1007/s11596-012-0082-y

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  • DOI: https://doi.org/10.1007/s11596-012-0082-y

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