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Silver nanoparticles-β-cyclodextrin-graphene nanocomposites based biosensor for guanine and adenine sensing

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Abstract

Graphene deposited with silver nanoparticles (AgNPs-β-CD-Gr) has been synthesized by thermal reduction of graphene oxide and silver nitrate via β-cyclodextrin (β-CD). It offers a simple, green, and efficient procedure to yield nanocomposites with silver nanoparticles anchored on graphene support, uniformly and stably. Guanine and adenine were simultaneously detected on the modified electrode (AgNPs-β-CD-Gr/GCE) by differential pulse voltammograms. The AgNPs-β-CD-Gr/GCE obviously improved the voltammetric response of guanine and adenine demonstrating that the synergistic effect of silver nanoparticles and graphene can significantly enhance the detecting sensitivity of guanine and adenine. The electrochemical biosensor based on AgNPs-β-CD-Gr exhibits wide linear ranges of 0.3–200 and 0.5–250 μM with detection limits of 0.09 and 0.15 μM for guanine and adenine, respectively. The proposed method was also applied for the measurement of trace-level of these purine bases in herring sperm DNA (dsDNA). The results suggest that the nanocomposite has a potential application for electrocatalytic biosensing.

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Acknowledgments

This work was financially supported by the National Nature Science Foundation of China (No. 51372206), Graduate Starting Seed Fund of Northwestern Polytechnical University (Z2013150 and Z2014022), and Doctorate Innovation Foundation of Northwestern Polytechnical University (CX201321).

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Authors and Affiliations

  1. Key Laboratory of Polymer Science and Technology, Shaanxi province, School of Science, Northwestern Polytechnical University, Xi’an, 710129, China

    Yuchen Hui, Xiaoyan Ma, Xiuzhang Hou, Fang Chen & Jie Yu

  2. Key Laboratory of Space Applied Physics and Chemistry, Ministry of Education, Shaanxi province, School of Science, Northwestern Polytechnical University, Xi’an, 710129, China

    Yuchen Hui, Xiaoyan Ma, Xiuzhang Hou, Fang Chen & Jie Yu

Authors
  1. Yuchen Hui
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  2. Xiaoyan Ma
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  3. Xiuzhang Hou
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  5. Jie Yu
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Correspondence to Xiaoyan Ma.

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Hui, Y., Ma, X., Hou, X. et al. Silver nanoparticles-β-cyclodextrin-graphene nanocomposites based biosensor for guanine and adenine sensing. Ionics 21, 1751–1759 (2015). https://doi.org/10.1007/s11581-014-1343-5

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  • DOI: https://doi.org/10.1007/s11581-014-1343-5

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