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Three new species of Ophiocordyceps and overview of anamorph types in the genus and the family Ophiocordyceptaceae

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Abstract

Three new Ophiocordyceps species from Japan are described: 1) O. coenomyia, which is parasitic to awl-fly larvae; 2) O. arborescens; and 3) O. macroacicularis, which parasitize moth larvae. Ophiocordyceps coenomyia is characterized by a globose, alutaceous fertile part at the apex of cylindrical, luteofulvous-to-pale yellow stroma, immersed lanceolate perithecia, and ascospores dividing into partspores. Based on its morphology and phylogenetic position, the new species is closely related to O. heteropoda. The latter two species produce superficial oval perithecia at the apex of cylindrical stroma, multiseptate ascospores, and Hirsutella-type anamorphs on the culture media. However, they are distinguished by the shape and size of their stroma. Multi-locus sequence analysis revealed that O. arborescens and O. macroacicularis were in the same phylogenetic clade, which is a sister to the clade containing O. sinensis. The distribution of anamorphic types in Ophiocordyceps is discussed.

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Acknowledgments

We thank Prof. Saigusa (Kyushu University, Japan), Prof. Nakamuta and Assoc. Prof. Nomura (Chiba University, Japan), Mr. Ohtake, Mr. Yamamoto, and Mr. Shimada for the identification of insects, as well as Mr. Kato and Mr. Ito for supplying fresh specimens. We also thank Ms. Tabuchi, Ms. Shimamura, Ms. Toyama, and Ms. Genra for sequencing assistance.

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Authors and Affiliations

  1. Biological Resource Center (NBRC), National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba, 292-0818, Japan

    Sayaka Ban

  2. Takatsuki City, Osaka, Japan

    Takeshi Sakane

  3. Fungus/Mushroom Resource and Research Center, Faculty of Agriculture, Tottori University, 4-101, Koyama minami, Tottori, 680-8553, Japan

    Akira Nakagiri

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  1. Sayaka Ban
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  2. Takeshi Sakane
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  3. Akira Nakagiri
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Correspondence to Sayaka Ban.

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Supplementary Fig. S1

Growth effect by five media; potato sucrose agar (PSA), molt extract agar (MA), trehalose peptone yeast extract agar (TrePY), respectively. All plates were incubated 14 days at 25 °C, in the dark. Bars indicate standard deviation (PPTX 50 kb)

Supplementary Fig. S2

Mycelial growth of several pH conditions on MA medium, which were incubated 14 days at 25 °C, in the dark (PPTX 50 kb)

Supplementary Fig. S3

Growth–temperature relationships of Ophiocordyceps coenomyia, O. arborescens and O. macroacicularis. Three media; PSA indicated by circle (○), MA by square (■) and TrePY by triangle (▲) were tested for three species (PPTX 65 kb)

Supplementary Fig. S4

Phylogenetic tree of Ophiocordyceps coenomyia sp. nov. and related species based on ITS sequences using NJ analysis. Numbers on the branches represent bootstrap values obtained from 1,000 replications (only values greater than 50 % are shown) (PPTX 64 kb)

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Ban, S., Sakane, T. & Nakagiri, A. Three new species of Ophiocordyceps and overview of anamorph types in the genus and the family Ophiocordyceptaceae. Mycol Progress 14, 1017 (2015). https://doi.org/10.1007/s11557-014-1017-8

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