Skip to main content
SpringerLink
Log in

Morphological and molecular evidence for a new species of Rhodotus from tropical and subtropical Yunnan, China

  • Original Article
  • Published:
Mycological Progress Aims and scope Submit manuscript

Abstract

Rhodotus has been regarded as a monotypic genus, consisting of only one species, R. palmatus, for a long time. Morphological and phylogenetic studies were carried out on collections of Rhodotus from temperate, subtropical and tropical China. Our phylogenetic analysis of DNA sequences of three loci (the internal transcribed spacer, the large subunit nuclear ribosomal RNA, and the translation elongation factor-1 alpha) revealed that there are two phylogenetic species in the northern hemisphere, which is in concordance with morphological traits, supporting the division of Rhodotus into two distinct species. Rhodotus asperior is described as a new species that differs phenotypically from R. palmatus in its broadly ellipsoid to subglobose, more roughened basidiospores, longer cheilocystidia with slightly thickened wall, and its occurrence in tropical and subtropical environments. The discovery of this new taxon indicates Rhodotus has a wider distribution than previously thought.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Binder M, Hibbett DS, Wang Z, Farnham WF (2006) Evolutionary relationships of Mycaureola diseae (Agaricales), a basidiomycete pathogen of a subtidal rhodophyte. Am J Bot 93:547–556

    Article  PubMed  Google Scholar 

  • Bulliard P (1785) Herbier de la France, Paris

  • Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf material. Phytochem Bull 19:11–15

    Google Scholar 

  • ECCF (European Council for the Conservation of Fungi) (2005) Country reports for the period 2000–2005:1–47

  • Edgar RC (2004) Muscle: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32:1792–1797

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analyses program for windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  • Han SK, Park YJ, Choi SK, Lee JO, Choi JH, Sung JM (2006) Some unrecorded higher fungi of the Seoraksan and Odaesan National Parks. Mycobiology 34:56–60

    Article  PubMed Central  PubMed  Google Scholar 

  • He X (1992) A new species and a new record of Tricholomataceae from China. Acta Mycol Sin 11:18–22 (In Chinese)

    Google Scholar 

  • Horak E (1968) Synopsis generum agaricalium (Die Gattungstypen der Agaricales). Beitr Kryptog Schweiz 13:1–741

    Google Scholar 

  • Imai S (1938) Studies on the Agaricaceae of Hokkaido II. J Fac Agric Hokkaido Imp Univ Sapporo 43:179–378

    Google Scholar 

  • Kornerup A, Wanscher JH (1981) Taschenlexikon der Farben, 3rd edn. Muster-Schmidt Verlag, Göttingen

    Google Scholar 

  • Krieglsteiner GJ (1979) Über einige Neufunde von Asco- und Basidiomycetes in der Bundesrepublik Deutschland. Z Mykol 45:35–44

    Google Scholar 

  • Kühner R, Romagnesi H (1984) Flore analytique des champignons supérieurs (4th printing). Masson, Paris

    Google Scholar 

  • Maire R (1926) Études mycologiques, fasc. 2. Bull Soc Mycol Fr 40:293–317

    Google Scholar 

  • Moncalvo JM, Vilgalys R, Redhead SA et al (2002) One hundred and seventeen clades of euagarics. Mol Phylogenet Evol 23:357–400

    Article  CAS  PubMed  Google Scholar 

  • Mueller GM (1992) Systematics of Laccaria (Agaricales) in the continental United States and Canada, with discussion on extralimital taxa and descriptions of extant types. Fieldiana Bot 30:1–158

    Google Scholar 

  • Noordeloos ME (1995) Tribus Rhodoteae. In: Bas C, Kuyper TH W, Noordeloos ME, Vellinga EC (eds) Flora agaricina neerlandica, vol 3. Balkema, Tokyo, pp 175–176

    Google Scholar 

  • Pegler DN, Young TWK (1975) Basidiospore form in the British species of Clitopilus, Rhodocybe and Rhodotus. Kew Bull 30:19–32

    Article  Google Scholar 

  • Pouchet MA (1932) Considérations sur Rhodotus palmatus (Bull., Fries) R. Maire, et sur ses variations. Bull Soc Mycol Fr 48:76–83

    Google Scholar 

  • Redhead SA (1989) A biogeographical overview of the Canadian mushroom flora. Can J Bot 67:3003–3062

    Article  Google Scholar 

  • Rehner SA (2001) Primers for elongation factor 1-α (EF1-α). http://www.aftol.org/pdfs/EF1primer.pdf

  • Rehner SA, Buckley E (2005) A Beauveria phylogeny inferred from nuclear ITS and EF1-sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 97:84–98

    Article  CAS  PubMed  Google Scholar 

  • Ripkova S (2003) New, rare and less known macromycetes in Slovakia I. Czech Mycol 55:187–200

    Google Scholar 

  • Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–166574

    Article  CAS  PubMed  Google Scholar 

  • Sundberg WJ, Methven AS, Monoson HL (1997) Rhodotus palmatus (Basidiomycetes, Agaricales, Tricholomataceae) in Illinois. Mycotaxon 65:403–410

    Google Scholar 

  • Swofford DL (2004) PAUP*. Phylogenetic analysis using parsimony (* and other methods), Version 4.01. Sinauer, Sunderland

    Google Scholar 

  • Taylor JW, Jacobson DJ, Kroken S, Kasuga T, Geiser DM, Hibbett DS, Fisher MC (2000) Phylogenetic species recognition and species concepts in fungi. Fungal Genet Biol 31:21–32

    Article  CAS  PubMed  Google Scholar 

  • Thiers B [continuously updated]. Index Herbariorum: A global directory of public herbaria and associated staff. New York Botanical Garden’s Virtual Herbarium. http://sweetgum.nybg.org/ih/

  • Tolgor B, Fan YG (2008) Study on domestication of Rhodotus palmatus. Edible Fungi China 27(5):16–18

    Google Scholar 

  • Vilgalys R, Hester M (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol 172:4238–4246

    CAS  PubMed Central  PubMed  Google Scholar 

  • Wang B, Zhang MJ (1992) New record of Rhodotus palmatus from China. Microbiology 19:72 (In Chinese)

    Google Scholar 

  • Wang L, Yang ZL, Liu JH (2004) Two new species of Laccaria (Basidiomycetes) from China. Nova Hedwig 79:511–517

    Article  Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenies. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: A guide to methods and applications. Academic, San Diego, pp 315–322

    Google Scholar 

Download references

Acknowledgments

The authors are very grateful to the following people: Dr. J. Borovička, Department of Nuclear Spectroscopy, Nuclear Physics Institute, the Academy of Sciences of the Czech Republic, for sending us a collection of R. palmatus; Dr. E. Horak, Innsbruck, Austria, for giving us good suggestion for the line-drawings; Dr. R.J. Vilgalys, Duke University, USA, for supplying accurate information about the GenBank sequence of the North American R. palmatus; Dr. Y.C. Li, Kunming Institute of Botany (KIB), Chinese Academy of Sciences (CAS), for providing valuable images of R. asperior; Dr. Z.X Ren and Mr. G. Wu, KIB, for helping us in using the SEM facility. The authors thank Dr. Hong Luo (DOE-Plant Research Laboratory, Michigan State University, USA) for polishing the English. This work was supported by the National Natural Science Foundation of China (No. 31170024), the Ministry of Science and Technology of China (2008FY110300), and the Knowledge Innovation Program of the CAS (No. KSCX2-EW-Z-9).

Author information

Authors and Affiliations

  1. Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, China

    Li-Ping Tang, Yan-Jia Hao, Qing Cai & Zhu L. Yang

  2. School of Pharmacology, Kunming Medical University, Kunming, 650221, China

    Li-Ping Tang

  3. Institute of Mycology, Jilin Agriculture University, Changchun, 130118, China

    Bau Tolgor

  4. University of Chinese Academy of Sciences, Beijing, 100039, China

    Li-Ping Tang, Yan-Jia Hao & Qing Cai

Authors
  1. Li-Ping Tang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yan-Jia Hao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Qing Cai
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Bau Tolgor
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Zhu L. Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhu L. Yang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tang, LP., Hao, YJ., Cai, Q. et al. Morphological and molecular evidence for a new species of Rhodotus from tropical and subtropical Yunnan, China. Mycol Progress 13, 45–53 (2014). https://doi.org/10.1007/s11557-013-0890-x

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11557-013-0890-x

Keywords

Search

Navigation