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Alternative splicing switching in stem cell lineages

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Frontiers in Biology

Abstract

The application of stem cells to regenerative medicine depends on a thorough understanding of the molecular mechanisms underlying their pluripotency. Many studies have identified key transcription factor-regulated transcriptional networks and chromatin landscapes of embryonic and a number of adult stem cells. In addition, recent publications have revealed another interesting molecular feature of stem cells—a distinct alternative splicing pattern. Thus, it is possible that both the identity and activity of stem cells are maintained by stem cell-specific mRNA isoforms, while switching to different isoforms ensures proper differentiation. In this review, we will discuss the generality of mRNA isoform switching and its interaction with other molecular mechanisms to regulate stem cell pluripotency, as well as the reprogramming process in which differentiated cells are induced to become pluripotent stem cell-like cells (iPSCs).

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  1. Systems Biology Center, National Heart, Lung and Blood Institute, NIH, Bethesda, MD, 20892, USA

    Iouri Chepelev

  2. Department of Biology, The Johns Hopkins University, 3400 North Charles Street, Baltimore, MD, 21218, USA

    Xin Chen

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Chepelev, I., Chen, X. Alternative splicing switching in stem cell lineages. Front. Biol. 8, 50–59 (2013). https://doi.org/10.1007/s11515-012-1198-y

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