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Double signal amplification sandwich-structured immunosensor based on TiO2 nanoparticles enhanced CdSe@ZnS QDs electrochemiluminescence and the dramatic quenching effect of Au@polydopamine nanoparticles

基于TiO2增强效应以及聚多巴胺修饰的金纳米粒子高效电致化学发光猝灭的双信号放大对癌胚抗原CEA的检测

  • Article
  • Chemistry
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Science Bulletin

Abstract

In this work, a double signal amplified immunosensor based on the enhanced CdSe@ZnS quantum dots (QDs) electrochemiluminescence (ECL) via TiO2 nanoparticles (TiO2 NPs) and the outstanding quencher of polydopamine (PDA) decorated Au nanoparticles (Au@PDA NPs) for ultrasensitive detection of carcinoembryonic antigen (CEA) has been successfully achieved. The ECL of CdSe@ZnS QDs with different sizes has been investigated carefully, especially cooperation with TiO2 NPs. Au@PDA NPs have been synthesized and characterized by transmission electron microscopy (TEM) and UV–Vis spectrum, which acted as ECL quenchers to label the secondary antibody (Ab2) of CEA to form Ab2/Au@PDA NPs conjugates. The sandwich-structured immunosensor was formed between capture antibody (Ab1) on CdSe@ZnS QDs/TiO2 NPs/glassy carbon electrode, CEA and Ab2/Au@PDA NPs conjugates, resulting in a proportional ECL quenching signal relevant to the CEA concentration. Thus, CEA as a model biomarker has been detected in the linear range from 0.001 to 100 ng mL−1 with a limit of detection of 0.35 pg mL−1 (S/N = 3).

摘要

研究TiO2增强效应以及聚多巴胺(PDA)修饰的金纳米粒子(Au@PDA NPs)对CdSe@ZnS核壳结构量子点电化学发光信号的猝灭作用。基于此本文设计了一种双信号放大电化学发光(ECL)癌胚抗原(CEA)免疫传感器,测试TiO2对不同尺寸CdSe@ZnS量子点的增强效果。结果表明,TiO2对尺寸大的量子点ECL增强效果比尺寸小的量子点高。Au@PDA NPs表现出对电致化学发光高效的猝灭效应。通过TiO2增强及Au@PDA NPs猝灭效应的双信号放大,可得到CEA的检测线性范围为0.001~100 ng mL−1,检测线为0.35 pg mL−1(S/N = 3)。

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (21575022, 21535003), the National High Technology Research and Development Program of China (2015AA020502), the Open Research Fund of Key Laboratory of Energy Thermal Conversion and Control of Ministry of Education, Southeast University, and the Fundamental Research Funds for the Central Universities (KYLX15-0127).

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The authors declare that they have no conflict of interest.

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  1. School of Chemistry and Chemical Engineering, Southeast University, Nanjing, 211189, China

    Qi-Le Li & Shou-Nian Ding

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  1. Qi-Le Li
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Correspondence to Shou-Nian Ding.

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Li, QL., Ding, SN. Double signal amplification sandwich-structured immunosensor based on TiO2 nanoparticles enhanced CdSe@ZnS QDs electrochemiluminescence and the dramatic quenching effect of Au@polydopamine nanoparticles. Sci. Bull. 61, 931–938 (2016). https://doi.org/10.1007/s11434-016-1097-8

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  • DOI: https://doi.org/10.1007/s11434-016-1097-8

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