Research Paper

Science China Life Sciences

, Volume 55, Issue 6, pp 516-520

Open Access This content is freely available online to anyone, anywhere at any time.

Bivariate genome-wide association study suggests that the DARC gene influences lean body mass and age at menarche

  • Rong HaiAffiliated withCenter of System Biomedical Sciences, University of Shanghai for Science and TechnologyThe Affiliated Hospital of Inner Mongolia Medical College
  • , Lei ZhangAffiliated withCenter of System Biomedical Sciences, University of Shanghai for Science and TechnologyDepartment of Biostatistics, Tulane University
  • , YuFang PeiAffiliated withCenter of System Biomedical Sciences, University of Shanghai for Science and TechnologyDepartment of Biostatistics, Tulane University
  • , LanJuan ZhaoAffiliated withDepartment of Biostatistics, Tulane University
  • , Shu RanAffiliated withCenter of System Biomedical Sciences, University of Shanghai for Science and Technology
  • , YingYing HanAffiliated withCenter of System Biomedical Sciences, University of Shanghai for Science and Technology
  • , XueZhen ZhuAffiliated withCenter of System Biomedical Sciences, University of Shanghai for Science and Technology
  • , Hui ShenAffiliated withDepartment of Biostatistics, Tulane University
  • , Qing TianAffiliated withDepartment of Biostatistics, Tulane University
    • , HongWen DengAffiliated withCenter of System Biomedical Sciences, University of Shanghai for Science and TechnologyDepartment of Biostatistics, Tulane University Email author 

Abstract

Lean body mass (LBM) and age at menarche (AAM) are two important complex traits for human health. The aim of this study was to identify pleiotropic genes for both traits using a powerful bivariate genome-wide association study (GWAS). Two studies, a discovery study and a replication study, were performed. In the discovery study, 909622 single nucleotide polymorphisms (SNPs) were genotyped in 801 unrelated female Han Chinese subjects using the Affymetrix human genome-wide SNP array 6.0 platform. Then, a bivariate GWAS was performed to identify the SNPs that may be important for LBM and AAM. In the replication study, significant findings from the discovery study were validated in 1692 unrelated Caucasian female subjects. One SNP rs3027009 that was bivariately associated with left arm lean mass and AAM in the discovery samples (P=7.26×10−6) and in the replication samples (P=0.005) was identified. The SNP is located at the upstream of DARC (Duffy antigen receptor for chemokines) gene, suggesting that DARC may play an important role in regulating the metabolisms of both LBM and AAM.

Keywords

bivariate genome-wide association study age at menarche lean body mass DARC gene