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Unmodified and positively charged gold nanoparticles for sensitive colorimetric detection of folate receptor via terminal protection of small molecule-linked ssDNA

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Abstract

The detection of protein/small molecule interactions plays important roles in drug discovery and protein/metabolite interactions in biology. In this work, by coupling the terminal protection of small molecule-linked ssDNA strategy with the unmodified and positively charged gold nanoparticle ((+)AuNP) nanoprobes, we have developed a sensitive and simple colorimetric sensor for the detection of folate receptor, a highly expressed protein in many kinds of malignant tumors. The target folate receptor binds the folate moieties of the folate-linked ssDNA through high affinity interactions and protects the protein-bound ssDNA from digestion by exonuclease I. The protected ssDNA thus adsorbs the ((+)AuNP) through electrostatic interactions, leading to a red-to-blue color change of the sensing solution for sensitive colorimetric detection of folate receptor at the sub-nanomolar level. Besides, this colorimetric sensor shows high selectivity toward folate receptor against other control proteins. The developed sensor avoids the modification/conjugation of the AuNP nanoprobes and the involvement of any expensive instruments for signal transduction in protein detection. Featured with these obvious advantages, the colorimetric sensor strategy demonstrated herein can be easily expanded for sensitive and convenient detection of various protein/small molecule interactions.

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Chongqing University of Technology, Chongqing, 400054, China

    Min Wang, Fangzhen Li, Bingying Jiang, Junqiang Xu & Shuo Li

  2. School of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China

    Yun Xiang & Ruo Yuan

Authors
  1. Min Wang
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  2. Fangzhen Li
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  3. Bingying Jiang
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  4. Junqiang Xu
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  5. Shuo Li
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  6. Yun Xiang
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  7. Ruo Yuan
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Corresponding authors

Correspondence to Bingying Jiang or Shuo Li.

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Wang, M., Li, F., Jiang, B. et al. Unmodified and positively charged gold nanoparticles for sensitive colorimetric detection of folate receptor via terminal protection of small molecule-linked ssDNA. Sci. China Chem. 59, 770–775 (2016). https://doi.org/10.1007/s11426-016-5589-9

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  • DOI: https://doi.org/10.1007/s11426-016-5589-9

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