Abstract
Parkinson’s disease (PD) is a neurodegenerative disorder characterized by the irreversible loss of dopaminergic neurons in the nigrostriatal pathway with subsequent dopamine deficiency. Environmental causes have been proposed through molecules, such as 1-methyl-4-phenylpyridinium (MPP+), to induce oxidative stress. The methanolic extract of plants of the genus Buddleja has been reported to have in vitro and in vivo antioxidant properties to protect against neuronal death. In the present study, the neuroprotective effect of Buddleja cordata methanolic extract in the MPP+ PD rat model was investigated. Animals were administered orally with 50 or 100 mg/kg of methanolic extract every 24 h for 14 days. Twenty hours later, rats were infused with an intrastriatal stereotaxic microinjection of 10 µg MPP+ in 8 μl sterile saline solution. Six days later, the animals were treated with 1 mg/kg apomorphine to record ipsilateral rotations for 1 h. All the rats were killed by decapitation and the lesioned striatum was dissected for dopamine and lipid peroxidation quantifications. Both methanolic extract doses led to a significantly lower (P < 0.05) number of ipsilateral rotations (75–80 %). This behavioral protection was corroborated with 60 % level of dopamine preservation (P < 0.05) and 90 % decrease in the formation of lipidic fluorescent products in the striatum (P < 0.05). This study demonstrates the antioxidant and neuroprotective effect of Buddleja cordata methanolic extract in the MPP+ PD rat model, possibly due to the involvement of phenylpropanoids.
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Abbreviations
- ME:
-
Methanolic extract
- MPP+ :
-
1-Methyl-4-phenylpyridinium
- PD:
-
Parkinson’s disease,
- MPTP:
-
1-Methyl-4-phenyl-1,2,3,6-tetrahydrophenylpyridine
- MAO-B:
-
Monoamine oxidase B
- DA:
-
Dopamine
References
Toulouse A, Sullivan A (2008) Progress in Parkinson’s disease—where do we stand? Prog Neurobiol 85:376–392
Giroux ML (2007) Parkinson disease: managing a complex, progressive disease at all stages. Cleve Clin J Med 74:313–328
Agid Y, Javoy-Agid F, Ruberg M (1987) Biochemistry of neurotransmitters in Parkinson’s disease. In: Marsden CE, Fahn S (eds) Movements disorders. Butterworth, London, pp 166–230
Blum D, Torch S, Lambeng N, Nissou M, Benabid A, Sadoul R, Jean-Marc V (2001) Molecular pathways involved in the neurotoxicity of 6-OHDA, dopamine and MPTP: contribution to the apoptotic theory in Parkinson’s disease. Prog Neurobiol 65:135–172
Boll MC, Alcaraz-Zubeldia M, Ríos C (2011) Medical management of Parkinson’s disease: focus on neuroprotection. Curr Neuropharmacol 192:350–359
Vauzour D, Rodriguez-Mateos A, Corona G, Oruna-Concha MJ, Spencer JPE (2010) Polyphenols and human health: prevention of disease and mechanisms of action. Nutrients 2:1106–1131
Lozoya X, Aguilar A, Camach JR (1987) Encuesta sobre el uso actual de plantas en la medicina tradicional. Rev Med Inst Mex Seguro Soc 25:283–291
Martínez-Vázquez M, Ramírez-Apan TO, Lastra AL, Bye R (1998) A comparative study of the analgesic and anti-inflammatory activities of pectolarin isolated from Cirsium subcoriaceum and linarin isolated from Buddleia cordata. Planta Med 64:134–137
Avila JG, de Liverant JG, Martínez A, Martínez G, Muñoz JL, Arciniegas A, Romo de Vivar A (1999) Mode of action of Buddleja cordata verbacoside against Staphylococcus aureus. J Ethnopharmacol 66:75–78
Rodríguez-Zaragoza S, Ordaz C, Avila G, Muñoz JL, Arciniegas A, Romo de Vivar A (1999) In vitro evaluation of the amebicidal activity of Buddleia cordata (Loganiaceae, H.B.K.) on several strains of Acanthamoeba. J Ethnopharmacol 66:327–334
Díaz BR, Jiménez M, Auró A (2000) Evaluación del efecto parasiticida de los extractos acuoso y metanólico de Buddleja cordata HBK (Tepozán) sobre Costia necatrix en tilapia (Oreochromis sp). Vet Mex 31:189–194
Lee DH, Ha N, Bu YM, Choi HI, Park YG, Kim YB, Kim MY, Kim H (2006) Neuroprotective effect of Buddleja officinalis extract on transient middle cerebral artery occlusion in rats. Biol Pharm Bull 29:1608–1612
Li YY, Lu JH, Li Q, Zhao YY, Pu XP (2008) Pedicularioside A from Buddleia lindleyana induced by 1-methyl-4-phenylpyridinium ions (MPP+) in primary cultures of rat mesencephalic neurons. Eur J Pharmacol 579:134–140
Avila JG, Castañeda CMC, Benitez FJC, Durán DA, Barroso VR, Martíınez CG, Romo de Vivar A (2005) Photoprotective activity of Buddleja scordioides. Fitoterapia 76:301–309
Backhouse N, Rosales L, Apablaza C, Goity L, Erazo S, Negrete R, Theodoluz C, Rodríguez J, Delporte C (2008) Analgesic, anti-inflammatory and antioxidant properties of Buddleja globosa Buddlejaceae. J Ethnopharmacol 116:263–269
Paxinos G, Watson C (1998) The rat brain in stereotaxic coordinates. Academic Press, New York, p 456
Schwarcz R, Fuxe K, Agnati LF, Hokfelt T, Coyle JT (1979) Rotational behaviour in rats with unilateral striatal kainic acid lesions: a behavioural model for studies on intact dopamine receptors. Brain Res 170:485–495
Alcaraz-Zubeldia M, Rojas P, Boll C, Rıos C (2001) Neuroprotective effect of acute and chronic administration of copper (II) sulfate against MPP+ neuro-toxicity in mice. Neurochem Res 26:61–66
Triggs WJ, Willmore LJ (1984) In vivo lipid peroxidation in rat brain following intracortical Fe2+ injection. J Neurochem 42:976–979
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin-phenol reagent. J Biol Chem 193:265–275
Díaz BR, Jiménez M, Auró A (2000) Evaluación del efecto parasiticida de los extractos acuoso y metanólico de Buddleja cordata HBK (Tepozán) sobre Costia necatrix en tilapia (Oreochromis sp). Vet Mex 31:189–194
Houghton PJ (1986) Ethnopharmacology of some Buddleja species. J Ethnopharmacol 11:293–308
Yamamoto A, Nitta S, Miyase T, Ueno A, Wu LJ (1993) Phenylethanoid and lignan-iridoid complex glycosides from roots of Buddleja davidii. Phytochemistry 32:421–425
Funes L, Fernández-Arroyo S, Laporta O, Pons A, Roche E, Segura-Carretero A, Fernández-Gutireez A, Micol V (2009) Correlation between plasma antioxidant capacity and verbascoside levels in rats after oral administration of lemon verbena extract. Food Chem 117:589–598
Pardo F, Perich F, Villarroel L, Torres R (1993) Isolation of verbascoside, an antimicrobial constituent of Buddleja globosa leaves. J Ethnopharmacol 139:221–223
Geng X, Tian X, Tu P, Pu X (2007) Neuroprotective effects of echinacoside in the mouse MPTP model of Parkinson’s disease. Eur J Pharmacol 564:66–74
Zhao Q, Gao J, Li W, Cai D (2010) Neurotrophic and neurorescue effects of Echinacoside in the subacute MPTP mouse model of Parkinson’s disease. Brain Res 346:224–236
Lee KJ, Woo ER, Choi CY, Shin DW, Lee DG, You HJ, Jeong HG (2004) Protective effect of acteoside on carbon tetrachloride-induced hepatotoxicity. Life Sci 74:1051–1064
Dostal V, Roberts CM, Link C (2010) Genetic mechanisms of coffee extract protection in a Caenorhabditis elegans model of b-amyloid peptide toxicity. Genetics 186:857–866
González-Correa JA, Navas MD, Lopez-Villodres JA, Trujillo M, Espartero JL, De la Cruz JP (2008) Neuroprotective effect of hydroxytyrosol and hydroxytyrosol acetate in rat brain slices subjected to hypoxia-reoxygeation. Neurosci Lett 446:143–146
Zhang J, Stanley RA, Melton LD (2006) Lipid peroxidation inhibition capacity assay for antioxidants based on liposomal membranes. Mol Nutr Food Res 50:714–724
Lankha PK, Lonsane BK (1997) Production and application of tannin acyl hydrolase. State of the art. Adv Appl Microbiol 44:215–260
Poquet L, Clifford MN, Williamson G (2008) Transport and metabolism of ferulic acid through the colonic epithelium. Drug Metab Dispos 36:190–197
Konishi Y, Kobayashi S (2004) Transepithelial transport of chlorogenic acid, caffeic acid, and their colonic metabolites in intestinal caco-2 cell monolayers. J Agric Food Chem 52:2518–2526
Watanabe H, Yashiro T, Tohjo Y, Konishi Y (2006) Non-involvement of the human monocarboxylic acid transporter 1 (MCT1) in the transport of phenolic acid. Biosci Biotechnol Biochem 70:928–1933
Manna C, Galletti P, Maisto G, Cucciolla V, D’Angelo S, Zappia V (2000) Transport mechanism and metabolism of olive oil hydroxytyrosol in Caco-2 cells. FEBS Lett 470:341–344
Tuck KL, Freeman MP, Hayball PJ, Stretch GL, Stupans I (2001) The in vivo fate of hydroxytyrosol and tyrosol, antioxidant phenolic constituents of olive oil, after intravenous and oral dosing of labeled compounds to rats. J Nutr 131:1993–1996
Vissers MN, Zock PL, Roodenburg AJ, Leenen R, Katan MB (2002) Olive oil phenols are absorbed in humans. J Nutr 132:409–417
Geellings A, López-Huerta EL, Morales JC, Boza J, Jiménez J (2003). Natural products and derivatives thereof for protection against neurodegenerative diseases. United States Patent Application Publication; US 2003/0236202 A1
Deiana M, Aruoma OI, Bianchi ML, Spencer JP, Kaur H, Halliwell B, Aeschbach R, Banni S, Dessi MA, Corongiu FP (1999) Inhibition of peroxynitrite dependent DNA base modification and tyrosine nitration by the extra virgin olive oil-derived antioxidant hydroxytyrosol. Free Radic Biol Med 26:762–769
Martin MA, Ramos S, Granado-Serrano AB, Rodriguez-Ramiro I, Trujillo M, Bravo L, Goya L (2010) Hydroxytyrosol induces antioxidant/detoxificant enzymes and Nrf2 translocation via ex- tracellular regulated kinases and phosphatidylinositol-3-kinase/protein kinase B pathways in HepG2 cells. Mol Nutr Food Res 54:956–966
Acknowledgments
This work has been partially supported by the spectrophotometry systems obtained from the CONACYT Grant 106436. The natural extract and the other material used in this research were supported by the DGAPA-UNAM, PAPIIT: IN219612. Pérez-Barrón received a fellowship from CONACYT 44067.
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Pérez-Barrón, G., Ávila-Acevedo, J.G., García-Bores, A.M. et al. Neuroprotective effect of Buddleja cordata methanolic extract in the 1-methyl-4-phenylpyridinium Parkinson’s disease rat model . J Nat Med 69, 86–93 (2015). https://doi.org/10.1007/s11418-014-0866-4
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DOI: https://doi.org/10.1007/s11418-014-0866-4