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Acylated phenylethanoid glycosides, echinacoside and acteoside from Cistanche tubulosa, improve glucose tolerance in mice

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Abstract

Acylated phenylethanoid glycosides, echinacoside (1) and acteoside (2), principal constituents in stems of Cistanche tubulosa (Orobanchaceae), inhibited the increase in postprandial blood glucose levels in starch-loaded mice at doses of 250–500 mg/kg p.o. These compounds (1 and 2) also significantly improved glucose tolerance in starch-loaded mice after 2 weeks of continuous administration at doses of 125 and/or 250 mg/kg/day p.o. without producing significant changes in body weight or food intake. In addition, several constituents from C. tubulosa, including 1 (IC50 = 3.1 μM), 2 (1.2 μM), isoacteoside (3, 4.6 μM), 2′-acetylacteoside (4, 0.071 μM), tubulosides A (5, 8.8 μM) and B (9, 4.0 μM), syringalide A 3-O-α-l-rhamnopyranoside (10, 1.1 μM), campneoside I (13, 0.53 μM), and kankanoside J1 (14, 9.3 μM), demonstrated potent rat lens aldose reductase inhibitory activity. In particular, the potency of compound 4 was similar to that of epalrestat (0.072 μM), a clinical aldose reductase inhibitor.

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Acknowledgments

This work was supported in part by a Grant-in Aid for Scientific Research by Japan Society for the Promotion of Science (JSPS) KAKENHI a Grant Number 24590153 and The Japan–China Medical Association for the financial support.

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Authors and Affiliations

  1. Pharmaceutical Research and Technology Institute, Kinki University, 3-4-1 Kowakae, Higashi-osaka, Osaka, 577-8502, Japan

    Toshio Morikawa, Kiyofumi Ninomiya, Mio Imamura, Junji Akaki, Shota Fujikura, Yingni Pan, Masayuki Yoshikawa & Osamu Muraoka

  2. Antiaging Centre, Kinki University, 3-4-1 Kowakae, Higashi-osaka, Osaka, 577-8502, Japan

    Toshio Morikawa, Kiyofumi Ninomiya & Osamu Muraoka

  3. School of Traditional Chinese Medicines, Shenyang Pharmaceutical University, 103 Wenhua Rd., Shenyang, 110016, People’s Republic of China

    Yingni Pan & Dan Yuan

  4. Xinjiang Institute of Chinese Materia Medica and Ethnodrug, 9 Xinmin Rd., Ürümqi, 830002, Xinjiang, People’s Republic of China

    Xiaoguang Jia

  5. Eishin Trading Co., Ltd., 2-13-4 Doshin, Kita-ku, Osaka, 530-0035, Japan

    Zheng Li

Authors
  1. Toshio Morikawa
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  2. Kiyofumi Ninomiya
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  3. Mio Imamura
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  9. Xiaoguang Jia
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Correspondence to Osamu Muraoka.

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Morikawa, T., Ninomiya, K., Imamura, M. et al. Acylated phenylethanoid glycosides, echinacoside and acteoside from Cistanche tubulosa, improve glucose tolerance in mice. J Nat Med 68, 561–566 (2014). https://doi.org/10.1007/s11418-014-0837-9

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  • DOI: https://doi.org/10.1007/s11418-014-0837-9

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