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Antifibrotic activity of coumarins from Cnidium monnieri fruits in HSC-T6 hepatic stellate cells

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Abstract

The CHCl3 fraction of Cnidium monnieri fruits significantly inhibited the proliferation of hepatic stellate cells in an in-vitro assay system employing HSC-T6 hepatic stellate cell lines. Activity-guided fractionation of the CHCl3 fraction of C. monnieri led to the isolation of ten coumarins: osthol (1), meranzin (2), auraptenol (3), meranzin hydrate (4), 7-hydroxy-8-methoxy coumarin (5), imperatorin (6), xanthotoxol (7), xanthotoxin (8), bergapten (9) and isopimpinellin (10). Of these, compounds 1 and 6 significantly inhibited proliferation of HSCs in a time- and concentration-dependent manner. In addition, compounds 1 and 6 significantly reduced collagen content in HSC-T6 cells.

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Acknowledgments

We thank Prof. S.L. Freidman (Columbia University, New York) for the kindly gift of the HSC-T6 cells. This work was supported by the Medical Research Center Program (2010-0029480), the Regional Core Research Program (Chungbuk BIT Research-Oriented University Consortium) and Basic Science Research Program (2010-0025054) through the National Research Foundation of Korea (NRF).

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Authors and Affiliations

  1. College of Pharmacy, Chungbuk National University, 410 Seongbong-ro, Heungduk-gu, Cheongju, 361-763, Korea

    Eunjin Shin, Chul Lee, Bang Yeon Hwang & Mi Kyeong Lee

  2. College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul, 151-742, Korea

    Sang Hyun Sung & Young Choong Kim

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  1. Eunjin Shin
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  2. Chul Lee
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  3. Sang Hyun Sung
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  4. Young Choong Kim
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  5. Bang Yeon Hwang
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  6. Mi Kyeong Lee
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Correspondence to Mi Kyeong Lee.

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Shin, E., Lee, C., Sung, S.H. et al. Antifibrotic activity of coumarins from Cnidium monnieri fruits in HSC-T6 hepatic stellate cells. J Nat Med 65, 370–374 (2011). https://doi.org/10.1007/s11418-010-0485-7

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  • DOI: https://doi.org/10.1007/s11418-010-0485-7

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