Skip to main content
SpringerLink
Log in

The effect of isosaponarin isolated from wasabi leaf on collagen synthesis in human fibroblasts and its underlying mechanism

  • Original Paper
  • Published:
Journal of Natural Medicines Aims and scope Submit manuscript

Abstract

Wasabi has been used as an important spice in Japanese foods. The wasabi leaves were used as a cosmetic material, but its biological activities have not yet been examined. We investigated the effect of isosaponarin derived from wasabi leaf on collagen synthesis in human fibroblasts. The production of type I collagen in human fibroblasts was increased with treatment of wasabi leaf extract. Isosaponarin isolated from wasabi leaves belonged to the group of flavone glycoside, and was the key compound in collagen synthesis from the wasabi leaf ingredients. Isosaponarin increased the type I collagen production at the mRNA gene level. The treatment of isosaponarin did not influence the production of transforming growth factor-β (TGF-β) protein, but increased the production of TGF-β type II receptor (TβR-II) protein and TβR-II mRNA. Prolyl 4-hydroxylase (P4H) protein and P4H mRNA were increased by treatment with isosaponarin. Heat shock protein 47 (HSP47) was not increased by treatment with isosaponarin. These results suggested that isosaponarin increased collagen synthesis in human fibroblasts, caused by up-regulated TβR-II and P4H production.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10
Fig. 11
Fig. 12
Fig. 13

Similar content being viewed by others

References

  1. Isshiki K, Tokuoka K, Mori R, Chiba S (1992) Preliminary examination of allyl isothiocyanate vapor for food preservation. Biosci Biotech Biochem 56(9):1476–1477

    Article  CAS  Google Scholar 

  2. Ono H, Tesaki S, Tanabe S, Watanabe M (1998) 6-Methylsulfinylhexyl isothiocyanate and its homologues as food-originated compounds with antibacterial activity against Escherichia coli and Staphylococcus aureus. Biosci Biotech Biochem 62(2):363–365

    Article  CAS  Google Scholar 

  3. Morimitsu Y, Hayashi K, Nakagawa Y, Horio F, Uchida K, Osawa T (2000) Antiplatelet and anticancer isothiocyanates in Japanese domestic horseradish, wasabi. Biofactors 13:271–276

    Article  CAS  PubMed  Google Scholar 

  4. Nagai M, Okunishi I (2009) The effect of wasabi rhizome extract on atopic dermatitis-like symptoms in HR-1 hairless mice. J Nutr Sci Vitaminol 55(2):195–200

    Article  CAS  PubMed  Google Scholar 

  5. Fuke Y, Haga Y, Ono H, Nomura T, Ryoyama K (1997) Anti-carcinogenic activity of 6-methylsulfinylhexyl isothiocyanate-, an active anti-proliferative principal of wasabi (Eutrema wasabi Maxim.). Cytotechnology 25:197–203

    Article  CAS  Google Scholar 

  6. Gottschalck TE, Bailey JE (2004) International cosmetic ingredient dictionary and handbook, 10th edn, vol 1, introduction INCI name monographs A–K. The Cosmetics, Toiletries and Fragrances Association, pp 1983

  7. Penttinen RP, Kobayashi S, Bornstein P (1988) Transforming growth factor β increases mRNA for matrix proteins both in the presence and in the absence of changes in mRNA stability. Proc Natl Acad Sci USA 85(4):1105–1108

    Article  CAS  PubMed  Google Scholar 

  8. Massagué J (1996) TGFβ signaling: receptors, transducers, and Mad proteins. Cell 85(7):947–950

    Article  PubMed  Google Scholar 

  9. Macías-Silva M, Abdollah S, Hoodless PA, Pirone R, Attisano L, Wrana JL (1996) MADR2 is a substrate of the TGFβ receptor and its phosphorylation is required for nuclear accumulation and signaling. Cell 87(7):1215–1224

    Article  PubMed  Google Scholar 

  10. Massagué J, Wotton D (2000) Transcriptional control by the TGF-β/Smad signaling system. EMBO J 19(8):1745–1754

    Article  PubMed  Google Scholar 

  11. Kivirikko KI, Myllyharju J (1998) Prolyl 4-hydroxylases and their protein disulfide isomerase subunit. Matrix Biol 16(7):357–368

    Article  CAS  PubMed  Google Scholar 

  12. Han XY, Wang W, Myllylä R, Virtanen P, Karpakka J, Takala TE (1999) mRNA levels for α-subunit of prolyl 4-hydroxylase and fibrillar collagens in immobilized rat skeletal muscle. J Appl Physiol 87(1):90–96

    CAS  PubMed  Google Scholar 

  13. Nagata K, Hosokawa N (1996) Regulation and function of collagen-specific molecular chaperone, HSP47. Cell Struct Funct 21(5):425–430

    Article  CAS  PubMed  Google Scholar 

  14. Nagai N, Hosokawa M, Itohara S, Adachi E, Matsushita T, Hosokawa N, Nagata K (2000) Embryonic lethality of molecular chaperone Hsp47 knockout mice is associated with defects in collagen biosynthesis. J Cell Biol 150(6):1499–1505

    Article  CAS  PubMed  Google Scholar 

  15. Obayashi K, Akamatsu H, Okano Y, Matsunaga K, Masaki H (2006) Exogenous nitric oxide enhances the synthesis of type I collagen and heat shock protein 47 by normal human dermal fibroblasts. J Dermatol Sci 41(2):121–126

    Article  CAS  PubMed  Google Scholar 

  16. Graham RC Jr, Karnovsky MJ (1966) The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14(4):291–302

    CAS  PubMed  Google Scholar 

  17. Hosoya T, Yun YS, Kunugi A (2005) Five novel flavonoids from Wasabia japonica. Tetrahedron 61:7037–7044

    Article  CAS  Google Scholar 

  18. Shim JS, Kwon YY, Hwang JK (2008) The effects of panduratin A isolated from Kaempferia pandurata on the expression of matrix metalloproteinase-1 and type-1 procollagen in human skin fibroblasts. Planta Med 74(3):239–244

    Article  CAS  PubMed  Google Scholar 

  19. Galicka A, Nazaruk J (2007) Stimulation of collagen biosynthesis by flavonoid glycosides in skin fibroblasts of osteogenesis imperfecta type I and the potential mechanism of their action. Int J Mol Med 20(6):889–895

    CAS  PubMed  Google Scholar 

  20. Stipcevic T, Piljac J, Vanden Berghe D (2006) Effect of different flavonoids on collagen synthesis in human fibroblasts. Plant Foods Hum Nutr 61(1):29–34

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Kinjirushi Co., Ltd., 2-61 Yahata-hontori, Nakagawa-ku, Nagoya, 454-8526, Japan

    Masashi Nagai, Keiko Akita, Kazuno Yamada & Isao Okunishi

Authors
  1. Masashi Nagai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Keiko Akita
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kazuno Yamada
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Isao Okunishi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Masashi Nagai.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nagai, M., Akita, K., Yamada, K. et al. The effect of isosaponarin isolated from wasabi leaf on collagen synthesis in human fibroblasts and its underlying mechanism. J Nat Med 64, 305–312 (2010). https://doi.org/10.1007/s11418-010-0412-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11418-010-0412-y

Keywords

Search

Navigation