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Apolipoprotein A-I attenuates renal ischemia/reperfusion injury in rats

  • Original Paper
  • Published:
Journal of Biomedical Science

Abstract

Apolipoprotein A-I (ApoA-I), the major protein component of serum high-density lipoprotein (HDL), exhibits its anti-inflammatory activity in inflammatory responses. As renal inflammation plays an important role in ischemia/reperfusion (I/R) injury of the kidney, the aim of this study was to investigate the beneficial effect of ApoA-I on renal I/R injury in rats and the underlined mechanism. Using rats subjected to renal I/R by occlusion of bilateral renal pedicles, we found that administration of ApoA-I significantly reduced serum creatinine levels, serum TNF-α and IL-1β levels as well as tissue myeloperoxidase (MPO) activity, compared with I/R controls. Moreover, ApoA-I treatment suppresses the expression of intercellular adhesion molecules-1 (ICAM-1) and P-selectin on endothelium, thus diminishing neutrophil adherence and the subsequent tissue injury. These results showed that ApoA-I reduced I/R-induced inflammatory responses, decreased renal microscopic damage and improved renal function. It seems likely that ApoA-I protects kidney from I/R injury by inhibiting inflammatory cytokines release and neutrophil infiltration and activation.

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Authors and Affiliations

  1. Department of Biochemistry, School of Pharmacy, Fudan University, P.O. Box 257, No. 138 Yixueyuan Road, Shanghai, 200032, China

    Ning Shi & Man-Ping Wu

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  1. Ning Shi
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  2. Man-Ping Wu
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Correspondence to Man-Ping Wu.

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Shi, N., Wu, MP. Apolipoprotein A-I attenuates renal ischemia/reperfusion injury in rats. J Biomed Sci 15, 577–583 (2008). https://doi.org/10.1007/s11373-008-9258-7

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  • DOI: https://doi.org/10.1007/s11373-008-9258-7

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