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Insights into the effects of Zn exposure on the fate of tylosin resistance genes and dynamics of microbial community during co-composting with tylosin fermentation dregs and swine manure

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Abstract

Though heavy metals are widely reported to induce antibiotic resistance propagation, how antibiotic resistance changes in response to heavy metal abundances remains unclearly. In this study, the tylosin fermentation dregs (TFDs) and swine manure co-composting process amended with two exposure levels of heavy metal Zn were performed. Results showed that the bioavailable Zn contents decreased 2.6-fold averagely, and the removal percentage of total tylosin resistance genes was around 23.5% after the co-composting completed. Furthermore, the tylosin resistance genes and some generic bacteria may exhibited a hormetic-like dose-response with the high-dosage inhibition and low dosage stimulation induced by bioavailable Zn contents during the co-composting process, which represented a beneficial aspect of adaptive responses to harmful environmental stimuli. This study provided a comprehensive understanding and predicted risk assessment for the Zn-contaminate solid wastes deposal and suggested that low levels of Zn or other heavy metals should receive more attention for their potential to the induction of resistance bacteria and propagation of antibiotic resistance genes.

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Data Availability

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

This work was financially supported by the National Natural Science Foundation of China (grant number 51678189).

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Author notes

  1. Bo Zhang and Qingbin Yuan contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Urban Water Resources and Environment, Harbin, 150090, China

    Bo Zhang & Peng Wang

  2. School of Environment, Harbin Institute of Technology, Harbin, 150090, China

    Bo Zhang & Peng Wang

  3. College of Environmental Science and Engineering, Nanjing Tech University, Nanjing, 211816, Jiangsu, China

    Qingbin Yuan

  4. College of Environment and Safety Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China

    Meng meng Wang

  5. Department of Civil and Environmental Engineering, Rice University, Houston, TX, 77005, USA

    Ruonan Sun

  6. College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, China

    Huiling Liu

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  1. Bo Zhang
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Contributions

BZ: Experiment design and operation, validation, visualization, data curation, software, writing (original) draft, writing (review), and editing

QB Y: Investigation, visualization, formal analysis, data curation, writing (review), and editing

MM W: Methodology, conceptualization, and data curation

RN S: Methodology and data curation

HL L: Resources, project administration, supervision, writing (review), and editing

PW*: Funding acquisition, resources, project administration, supervision, writing (review), and editing

All authors read and approved the final manuscript.

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Correspondence to Peng Wang.

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Zhang, B., Yuan, Q., Wang, M.m. et al. Insights into the effects of Zn exposure on the fate of tylosin resistance genes and dynamics of microbial community during co-composting with tylosin fermentation dregs and swine manure. Environ Sci Pollut Res 28, 14423–14433 (2021). https://doi.org/10.1007/s11356-020-11471-y

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